Plant Cell, Tissue and Organ Culture

, Volume 46, Issue 3, pp 227–235 | Cite as

Somatic embryogenesis and plant regeneration of pepper in liquid media

  • Saadet Buyukalaca
  • Ferda Mavituna
Original Research Paper

Abstract

A protocol was developed for regeneration of pepper (Capsicum annuum var. Ace) through somatic embryogenesis in liquid media. For embryogenic callus formation, mature zygotic embryo explants were used on basal Murashige and Skoog medium with 9.05 μM 2,4-dichlorophenoxyacetic acid and 3% sucrose. Embryogenic callus was transferred to liquid basal Murashige and Skoog medium with 4.52 μM 2,4-dichlorophenoxyacetic acid and 3% sucrose in order to increase the mass of the embryogenic culture. After pretreatment with potassium citrate, cells were placed into embryo initiation medium with 6 g l-1l-proline and a decreased (10 mM) ammonium concentration. Embryos were matured in 1.89 μM abscisic acid containing half-strength Murashige and Skoog medium and converted into plants bothin vivo andin vitro at up to a 97% efficiency.

Key words

Capsicum spp maturation zygotic embryo explant 

Abbreviations

ABA

abscisic acid

B5

Gamborg et al. (1968)

BA

6-benzylaminopurine

2,4-d

2,4-dichlorophenoxyacetic acid

IAA

indole 3-acetic acid

MS

Murashige and Skoog (1962)

NAA

naphthaleneacetic acid

pcv

packed cell volume

SH

Schenk and Hildebrandt (1972)

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Copyright information

© Kluwer Academic Publishers 1996

Authors and Affiliations

  • Saadet Buyukalaca
    • 1
  • Ferda Mavituna
    • 1
  1. 1.Department of Chemical EngineeringUniversity of Manchester Institute of Science and TechnologyManchesterUK

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