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Annals of Surgical Oncology

, Volume 1, Issue 6, pp 495–503 | Cite as

Immunocytochemical assay for androgen receptors in prostate cancer: A prospective study of 63 cases with long-term follow-up

  • Louis P. Pertschuk
  • Richard J. Macchia
  • Joseph G. Feldman
  • Katherine A. Brady
  • Michael Levine
  • Dong S. Kim
  • Karen B. Eisenberg
  • Evelyn Rainford
  • Gail S. Prins
  • Geoffrey L. Greene
Original Articles

Abstract

Background: Numerous problems are associated with biochemical androgen receptor (AR) assay performance and interpretation in prostatic cancer. The purpose of this study was to determine if a novel immunocytochemical AR assay performed on intact tissue sections would prove useful in prognosticating endocrine response and survival.

Methods: A prospective study was done on 63 prostatic carcinomas maintained in liquid nitrogen for over a decade. The study used the peroxidaseantiperoxidase system and a polyclonal anti-AR antibody.

Results: Marked tissue and cellular heterogeneity of nuclear AR was apparent. A cut-off of 10% AR-positive cells maximized assay prognostic efficiency. Frequency of positivity was 48% and correlated significantly with endocrine response (p=0.03), time to progression (p=0.0016), and survival (p=0.02), but not with grade, stage, or ethnicity.

Conclusions: This AR assay could be prognostically useful in the clinical management of prostate cancer and is suitable for use in the community hospital laboratory.

Key Words

Prostate cancer Androgen receptors Immunocytochemistry Prognostic data 

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Copyright information

© The Society of Surgical Oncology, Inc. 1994

Authors and Affiliations

  • Louis P. Pertschuk
    • 1
  • Richard J. Macchia
    • 2
  • Joseph G. Feldman
    • 3
  • Katherine A. Brady
    • 4
  • Michael Levine
    • 4
  • Dong S. Kim
    • 1
  • Karen B. Eisenberg
    • 1
  • Evelyn Rainford
    • 1
  • Gail S. Prins
    • 5
  • Geoffrey L. Greene
    • 6
  1. 1.From the Department of PathologyState University of New YorkBrooklyn
  2. 2.Department of UrologyState University of New YorkBrooklyn
  3. 3.Department of Preventive Medicine and Community HealthState University of New YorkBrooklyn
  4. 4.School of Medicine, Health Science CenterState University of New YorkBrooklyn
  5. 5.Department of Obstetrics and Gynecology, Humana Hospital-Michael ReeseUniversity of Illinois College of MedicineUSA
  6. 6.the Ben May Laboratory for Cancer ResearchUniversity of ChicagoChicagoUSA

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