Origin of the duplicated ribonuclease gene in guinea-pig: Comparison of the amino acid sequences with those of two close relatives: Capybara and cuis ribonuclease
- 26 Downloads
The amino acid sequences of the pancreatic ribonuclease from capybara (Hydrochoerus hydrochaeris) and cuis (Galea musteloides) were determined. Both species belong to the same superfamily of the hystricomorph rodents as the guinea-pig. In guineapig pancreas two ribonucleases are present as a result of a rencent gene duplication, but in capybara and cuis pancreas only one single ribonuclease has been found. A most parsimonious tree of ribonucleases indicates that the gene duplication leading to both guinea-pig ribonucleases occurred before the divergence of guineapig from capybara and cuis. This would mean that changes in expression of the ribonuclease genes have occurred in these taxa.
Cuis and capybara ribonuclease have no Asn-X-Ser/Thr sequences and are carbohydrate-free proteins. Capybara ribonculease has leucine at position 114, a position occupied by proline in thecis-configuration in bovine pancreatic ribonuclease.
Key wordsRNAse Amino acid sequence Gene duplication Guinea-pig
Unable to display preview. Download preview PDF.
- Beintema JJ (1980) Ribonuclease variation within the species. In: Peeters H (ed) Proc. 28th coll. protides biol. fluids. Pergamon Press, Oxford, pp 139–141Google Scholar
- Beintema JJ, Lenstra JA (1982) Evolution of mammalian pancreatic ribonucleases. In: Goodman M (ed) Macromolecular sequences in systematic and evolutionary biology. Plenum, New York, pp 43–73Google Scholar
- Beintema JJ, Lenstra JA, Fitch WM (1980) The molecular evolution of mammalian pancreatic ribonuclease. In: Peeters H (ed) Proc. 28th coll. protides biol. fluids. Pergamon Press, Oxford, pp 133–137Google Scholar
- Fitch WM (1971) Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416Google Scholar
- Goodman M, Czelusniak J, Moore GW, Romero-Herrara AE, Matsuda G (1979) Fitting the gene lineage into its species lineage, A parsimony strategy illustrated by cladograms constructed from globin sequences. Syst Zool 28:132–163Google Scholar
- Gray WR (1967) Dansyl chloride procedure. Methods Enzymol 11:139–151Google Scholar
- Morris D (1965) The Mammals. Hodder and Stoughton, LondonGoogle Scholar
- Richards FM, Wyckoff HW (1973) In: Philips DC, Richards FM (eds) Atlas of molecular structures in biology. Vol 1, Ribonuclease-S. Clarendon, OxfordGoogle Scholar
- Suzuki H, Greco L, Parente A, Farina B, La Montagna R, Leone E (1976) Bovine seminal ribonuclease. In: Dayhoff MO (ed) Atlas of protein sequence and structure. Vol 5, Suppl. 2. National Biomedical Research Foundation, Washington DC, p 93Google Scholar
- Van den Berg A, Van den Hende-Timmer L, Beintema JJ (1976) Isolation, properties and primary structure of coypu and chinchilla pancreatic ribonuclease. Biochim Biophys Acta 453:400–409Google Scholar