The biological identification of native and of cooked proteins

Summary

In rabbits specific precipitating sera of high titre (up to 1 : 30.000) can be induced with native as well as with cooked proteins by means of single subcutaneous injections with the protein concerned emulgated in vaseline-lanoline. In the experiments muscle protein of cattle and dog serum and hen albumen have been used. The proteins, either cooked or native, are dried, pulverized and emulgated as such in vaseline-lanoline on a water-bath of 40–50° C., adding if needed some saline or distilled water. The injection follows immediately. The precitating sera prepared against cooked proteins give a precipitation reaction with the homologous native protein as well as with the cooked protein which is dissolved in NaOH.