, Volume 43, Issue 9–10, pp 551–556 | Cite as

High-performance liquid chromatography of some estrogens on chemically bonded phases

  • S. M. Petrović
  • M. Ačanski
  • V. Pejanović
  • J. A. Petrovic
Short Communications


The chromatographic behaviour of 30 estradiol and estrone derivatives has been studied on diol, amino- and cyanopropyl silica gel stationary phases with non-aqueous mobile phases. The slopes of linear relationships between the logarithm of the retention factor and logarithm of the volume fractions of the polar components of the binary eluents have been calculated and are discussed in relation to the characteristics of the solute, eluent and stationary phase. An equation for testing the separation efficiency of stationary phases has been derived.

Key Words

Column liquid chromatography Chemically bonded silica gel Mixed solvents Steroids 


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Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1996

Authors and Affiliations

  • S. M. Petrović
    • 1
  • M. Ačanski
    • 1
  • V. Pejanović
    • 2
  • J. A. Petrovic
    • 3
  1. 1.Department of Analytical Chemistry, Faculty of TechnologyUniversity of Novi SadNovi SadYugoslavia
  2. 2.ICN Galenika, InstituteBeogradYugoslavia
  3. 3.Institute of Chemistry, Faculty of SciencesUniversity of Novi SadNovi SadYugoslavia

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