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Chromatographia

, Volume 43, Issue 9–10, pp 507–512 | Cite as

Urine clean-up method for determination of six arsenic species by LC-AAS involving microwave assisted oxidation and hydride generation

  • M. López-Gonzálvez
  • M. M. Gómez
  • M. A. Palacios
  • C. Cámara
Originals

Summary

An effective clean-up method based on the precipitation of most high molecular mass inorganic salts and organic compounds in ethanol at −15°C has been developed for the determination of arsenite, arsenate, monomethylarsonate (MMA), dimethylarsinate (DMA), arsenobetaine (AsB) and arsenocholine (AsC) in urine. Analyte recovery was close to 100%. The six species were separated on an anionic HPLC column and determined by microwave-assisted oxidation-hydride generation-atomic absorption spectrometry. The detection limits and relative standard deviation of the whole procedure were within the 8–15 μg L−1 and 2.5–5.3% ranges, respectively, which allows application to the analysis of urine of people exposed to arsenic.

Key Words

Column liquid chromatography Urine clean-up Arsenic speciation Microwave assisted oxidation Atomic absorption spectrometry 

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Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1996

Authors and Affiliations

  • M. López-Gonzálvez
    • 1
  • M. M. Gómez
    • 1
  • M. A. Palacios
    • 1
  • C. Cámara
    • 1
  1. 1.Departamento de Química Analítica, Facultad de QuímicaUniversidad Complutense de MadridMadridSpain

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