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Determination of free aliphatic aldehydes in plasma by high-performance liquid chromatography of the 1,3-cyclohexanedione derivatives

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Summary

A simple and sensitive method is proposed for the measurement of aldehydes in plasma. The plasma samples are treated with 1,3-cyclohexanedione (CHD) and after addition of the internal standard (the CHD derivative of octaldehyde) are analysed by reversed-phase high-performance liquid chromatography with an acetonitrile-water gradient as the mobile phase. Detection is by fluorimetry with excitation at 395 nm and emission at 457 nm. In plasma samples spiked with aldehydes, the calibration curves showed good linearity in the range 0–10 μg mL−1. The recoveries of aldehydes were quantitative; coefficients of within-day and between-day variation were less than 13% and 11%, respectively. Applications to human and some animal plasma samples are reported.

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Authors and Affiliations

  1. Analytical Research Laboratory, Tanabe Seiyaku Co. Ltd., 16-89, Kashima 3-chome, Yodogawa-ku, Osaka, Japan

    M. Matsuoka, N. Imado, T. Maki, K. Banno & T. Sato

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  1. M. Matsuoka
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  2. N. Imado
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  3. T. Maki
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  4. K. Banno
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  5. T. Sato
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Matsuoka, M., Imado, N., Maki, T. et al. Determination of free aliphatic aldehydes in plasma by high-performance liquid chromatography of the 1,3-cyclohexanedione derivatives. Chromatographia 43, 501–506 (1996). https://doi.org/10.1007/BF02293001

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  • DOI: https://doi.org/10.1007/BF02293001

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