Summary
Aspects of cracking and joining capillaries have been investigated. Capillary coupling was achieved using various methods. The most successful used hydrofluoric acid-etched capillaries to form male and female ends which were then joined together. This type of joint was used to connect sections of capillary of similar and different internal diameters with minimal loss in resolution, peak width and number of theoretical plates. (Uridine and hypoxanthine was used as a test mixture). For hypoxanthine on a 50 μm/50 μm etched joined capillary 10 cm from the detector window the number of theoretical plates was 96.6% of that for hypoxanthine on an unbroken capillary. Following the relative success of capillary joining, a coupled capillary flowcell (50 μm/200 μm) was produced and evaluated.
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Hows, M.E.P., Perrett, D. A technique for capillary joining in capillary electrophoresis. Chromatographia 43, 200–204 (1996). https://doi.org/10.1007/BF02292951
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DOI: https://doi.org/10.1007/BF02292951