Summary
Ready detection of lactulose in milk and in mixtures with other sugars is achieved by diluting the sample ten times with acetone and successive thin-layer chromatographic separation on silica gel-boric acid, developed with acetonitrile: water 50∶20. The method allows detection of up to 0.02% lactulose on total carbohydrates and permits a semi-quantitative estimation of lactulose in milk.
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Martinez-Castro, I., Olano, A. Ready detection of small amounts of lactulose in dairy products by thin-layer chromatography. Chromatographia 14, 621–622 (1981). https://doi.org/10.1007/BF02291098
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DOI: https://doi.org/10.1007/BF02291098