, Volume 14, Issue 11, pp 621–622 | Cite as

Ready detection of small amounts of lactulose in dairy products by thin-layer chromatography

  • I. Martinez-Castro
  • A. Olano


Ready detection of lactulose in milk and in mixtures with other sugars is achieved by diluting the sample ten times with acetone and successive thin-layer chromatographic separation on silica gel-boric acid, developed with acetonitrile: water 50∶20. The method allows detection of up to 0.02% lactulose on total carbohydrates and permits a semi-quantitative estimation of lactulose in milk.

Key Words

Milk Lactulose Thin-layer chromatography 


  1. [1]
    S. Adachi, S. Patton, J. Dairy Sci.44, 1375 (1961).Google Scholar
  2. [2]
    F. W. Bernhart, E. D. Gagliardi, R. C. Stribley, J. Dairy Sci.48, 399 (1965).Google Scholar
  3. [3]
    I. Martinez-Castro, A. Olano, Rev. Esp. Lecheria no 110, 213 (1978).Google Scholar
  4. [4]
    R. G. Hendrickse, M. A. W. Wooldridge, A. Russell, Brit. Med. J. 1194 (1977).Google Scholar
  5. [5]
    S. Zagrodzki, A. Kulagowska, B. Krol. Qual. Plantarum Mat. Veg.16, 102 (1968).Google Scholar
  6. [6]
    S. Adachi, Anal. Biochem.12, 137 (1965).Google Scholar
  7. [7]
    I. Martinez-Castro, A. Olano, Milchwiss.35, 5 (1980).Google Scholar
  8. [8]
    M. F. Laker, J. Chromatography163, 9 (1979).Google Scholar
  9. [9]
    M. Müller, J. Walker-Smith, D. H. Smerling, H. Ch. Curtius, A. Prader, Clin. Chim. Acta24, 45 (1969).Google Scholar
  10. [10]
    L. Mouillet, F. M. Luquet, J. F. Boudier, Lait57, 37 (1977).Google Scholar
  11. [11]
    V. Siegenthaler, W. Bitter, Mitt. Gebiete Lebensm. Hyg.68, 448 (1977).Google Scholar

Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1981

Authors and Affiliations

  • I. Martinez-Castro
    • 1
  • A. Olano
    • 1
  1. 1.Instituto de Quimica Orgánica General/Instituto de Fermentaciones Industriales C.S.I.C.Madrid-6Spain

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