Chromatographia

, Volume 14, Issue 11, pp 621–622 | Cite as

Ready detection of small amounts of lactulose in dairy products by thin-layer chromatography

  • I. Martinez-Castro
  • A. Olano
Originals

Summary

Ready detection of lactulose in milk and in mixtures with other sugars is achieved by diluting the sample ten times with acetone and successive thin-layer chromatographic separation on silica gel-boric acid, developed with acetonitrile: water 50∶20. The method allows detection of up to 0.02% lactulose on total carbohydrates and permits a semi-quantitative estimation of lactulose in milk.

Key Words

Milk Lactulose Thin-layer chromatography 

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Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1981

Authors and Affiliations

  • I. Martinez-Castro
    • 1
  • A. Olano
    • 1
  1. 1.Instituto de Quimica Orgánica General/Instituto de Fermentaciones Industriales C.S.I.C.Madrid-6Spain

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