Summary
The scope and limitations of the application of the commercially available cation-exchange thin-layer chromatoplate (Fixion 50-X8) for the resolution of nucleotide mixtures is presented. A possible separation mechanism is proposed and discussed.
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Abbreviations
- TLC:
-
thin-layer chromatography
- pA:
-
adenosine 5′-phosphate
- Ap:
-
adenosine 2′ (3′)-phosphate
- p<A:
-
adenosine 3′,5′-cyclic phosphate
- A>p:
-
adenosine 2′,3′-cyclic phosphate
- pC:
-
cytidine 5′-phosphate
- Cp:
-
cytidine 2′(3′)-phosphate
- p<C:
-
cytidine 3′,5′-cyclic phosphate
- C>p:
-
cytidine 2′,3′-cyclic phosphate
- pG:
-
guanosine 5′-phosphate
- Gp:
-
guanosine 2′(3′)-phosphate
- p<G:
-
guanosine 3′,5′-cyclic phosphate
- G>p:
-
guanosine 2′,3′-cyclic phosphate
- pI:
-
inosine 5′-phosphate
- Ip:
-
inosine 2′(3′)-phosphate
- p<I:
-
inosine 3′,5′-cyclic phosphate
- I>p:
-
inosine 2′,3′-cyclic phosphate
- pU:
-
uridine 5′-phosphate
- p<U:
-
uridine 3′,5′-cyclic phosphate
- d-pA:
-
2′-deoxyadenosine 5′-phosphate
- d-pC:
-
2′-deoxycytidine 5′-phosphate
- d-pG:
-
2′-deoxyguanosine 5′-phosphate
- pT:
-
thymidine 5′-phosphate
- aHCl :
-
activity of the developing HCl solution
References
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Publication No. 11 in the series dealing with the separation of nucleic acid bases, nucleosides and nucleotides on thin-layers consisting of strong cation-exchange resins.
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Tomasz, J. Scope, limitations and mechanism of nucleotide separation by thin-layer chromatography with strong cation-exchange resin containing layers. Chromatographia 13, 345–349 (1980). https://doi.org/10.1007/BF02290903
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DOI: https://doi.org/10.1007/BF02290903