Chromatographia

, Volume 17, Issue 4, pp 205–208 | Cite as

Reversed-phase high-performance liquid chromatographic separation of theα- andβ-isomers of aspartyl peptides

  • Heng-Liang Jin
  • Yao Lin
  • Chong-Xi Li
  • Yuen-Hwa Yieh
  • Ai-Hsueh Chi
  • Yong-Jun Lu
  • Cheng Zhao
  • Hua-Zhe Jin
  • Chi-Yi Hsing
Originals

Summary

A high-performance liquid chromatography method is described for the separation of the α- and β-isomers of aspartyl-containing peptides such as N-benzyloxycarbonyl methyl aspartate, protected sweet dipeptide and delta sleep inducing peptide (DSIP, a nonapeptide). The α- and β-isomers were separated on a column containing octadecyl silica bonded-phase packing under reversed-phase conditions using methanol-water as the mobile phase containing a small amount of acetic acid. The resolution achieved meets the requirements of qualitative and quantitative analysis. This method is also suited for the determination of intermediates in the aspartyl peptide synthesis.

Key Words

Liquid chromatography Reversed-phase chromatography Aspartyl peptides Peptide analysis 

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References

  1. [1]
    R. W. Frei, L. Michel, W. Santi, J. Chromatogr.126, 665 (1976).Google Scholar
  2. [2]
    J. J. Hansen, T. Greibrokk, B. L. Currie, K. N.-G. Johansson, K. Folkers, J. Chromatogr.135, 155 (1977).Google Scholar
  3. [3]
    J. A. Feldman, M. L. Cohn, D. Blair, J. Liquid Chromatogr.1 (6), 833 (1977).Google Scholar
  4. [4]
    K. Krummen, J. Liquid Chromatogr.3 (9), 1243 (1980).Google Scholar
  5. [5]
    R. F. Adams, J. Chromatogr. Sci.9, 1273 (1979).Google Scholar
  6. [6]
    J. N. Seiber, C. J. Nelson, J. M. Benson, J. Chromatogr. Sci.16, 45 (1981).Google Scholar
  7. [7]
    J. River, R. Burgus, Biological/Biomedical Applications of Liquid Chromatography,G. L. Hawk (Ed.), Volume 10, Marcel Dekker, New York, 1979, p. 147.Google Scholar
  8. [8]
    E. P. Kroeff, D. J. Pietrzyk, Anal. Chem.50, 1353 (1978).Google Scholar
  9. [9]
    C. Hunter, K. Sugden, J. G. Lloyd-Jones, J. Liquid Chromatogr.3 (9), 1335 (1980).Google Scholar
  10. [10]
    W. W. K. Fong, E. Grushka, Anal. Chem.50, 1154 (1978).Google Scholar
  11. [11]
    Chi-Ai Hsueh, Li-Chong Xi, Yieh-Yuen Hwa, Lin Yao, Lu Yong Jun, Hsing-Chi Yi, Scientia SinicaB8, 727 (1982) (Chinese Ed.).Google Scholar
  12. [12]
    M. Bodanszky, J. C. Tolle, S. S. Deshmane, A. Bodanszky. Int. J. Peptide Res.12, 57 (1978).Google Scholar
  13. [13]
    M. Monnier, L. Dudler, R. Gachter, P. F. Maier, H. J. Tobler, G. A. Schoenenberger, Experientia33, 548 (1977).Google Scholar
  14. [14]
    Chi-Ai Hsueh, Li-Chong Xi, Yieh-Yuen Hwa, Lin Yao, Lu Yong Jun, Hsing-Chi Yi, Liu-Shih Yih, Chang-Wen Yen, Wang-Tseh Shun, Tsi-Siu Chuch, Proceedings of the Seventh American Peptide Symposium,D. H. Rich, E. Gross (Eds.), Pierce Chemical Company, 1981, p. 52.Google Scholar
  15. [15]
    L. R. Snyder, J. Chromatogr. Sci.10, 200 (1972).Google Scholar

Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1983

Authors and Affiliations

  • Heng-Liang Jin
    • 1
  • Yao Lin
    • 1
  • Chong-Xi Li
    • 1
  • Yuen-Hwa Yieh
    • 1
  • Ai-Hsueh Chi
    • 1
  • Yong-Jun Lu
    • 1
  • Cheng Zhao
    • 1
  • Hua-Zhe Jin
    • 1
  • Chi-Yi Hsing
    • 1
  1. 1.Department of ChemistryBeijing UniversityBeijingPeople's Republic of China

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