Summary
The liquid chromatographic behaviour of aliphatic mono- and polyamines belonging to different homologous series has been investigated using various pre-column derivatizing agents (OPA and CNB), mobile phases (water-MeOH and water-ACN), RP columns (different packing sizes and shapes) and detectors (UV and fluorescence) in order to characterize the different contributions to retention and the different selectivities under the conditions investigated. The parameters derived allowed computation of capacity factors in good agreement with experiments. The chromatographic method optimized with respect to OPA derivatization has been successfully utilized for the determination of putrescine, cadaverine and 2-phenyl-ethylamine in cheese samples under different ageing conditions.
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Mentasti, E., Sarzanini, C., Abollino, O. et al. Chromatographic behaviour of homologous amines: Comparison of derivatizing agents, columns and mobile phases. Chromatographia 31, 41–49 (1991). https://doi.org/10.1007/BF02290494
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DOI: https://doi.org/10.1007/BF02290494