Summary
The effects of eluent pH and organic modifier concentration on the capacity factor (k′) and selectivity of dipeptide isomers were investigated. It has been observed that the variation in the logarithm of the capacity factor of the dipeptide isomers is linearly dependent on the organic modifier concentration (Cb), however, the selectivity is almost independent of it. Both capacity factor and selectivity were seriously affected by the pH of the eluent. Both the capacity factor and the intercept of the ln k′ vs. Cb plot increased with increasing van der Waals volume of the non-polar amino acid subunit of the dipeptides.
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Zou, H.F., Zhang, Y.K., Dong, L.F. et al. Retention behavior of dipeptide isomers in reversed-phase high-performance liquid chromatography. Chromatographia 31, 27–30 (1991). https://doi.org/10.1007/BF02290492
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DOI: https://doi.org/10.1007/BF02290492