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Chromatographia

, Volume 25, Issue 8, pp 717–720 | Cite as

Quantitative determination of miconzole in human serum by high-performance liquid chromatography

  • H. Hosotsubo
Originals

Summary

A high-performance liqid chromatographic method is reported for the measurement of miconazole in systemic, fungal infectious patients. Pharmacokinetic data are presented for a single patient receiving miconazole therapy. Sample preparation involves protein precipitation by acetonitrile (1:1, vol/vol). Analyses are carried out on a reversed-phae chromatographic system using octadecylsilane stationary phase: a mobile phase consisting of 0.05 M acetate buffer (pH 7.4)acetonitrile (20:80, vol/vol) is used to elaute miconazole is quantified on the basis of ultraviolet absorption at 220 nm. The precision of the method ranged from 3.21% at 0.5 mg/L to 0.85% at 2.0 mg/L. The limit of quantification was established as 0.1 mg/L. Interference from other drugs that are co-adimistered such as amphotericin B, 5-fluorocytosine of ketoconazole and most other comonly encountered drugs was not observed.

Key Words

Column liquid chromatography Reversed-phase chromtography Antifungla agent Pharmacokinetics 

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Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1988

Authors and Affiliations

  • H. Hosotsubo
    • 1
  1. 1.Central Laboratory for Clinicla InvestigationOsaka University HospitalOsakaJapan

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