, Volume 34, Issue 1–2, pp 79–82 | Cite as

Solid phase extraction conditions for the selective isolation of drugs from biological fluids predicted using liquid chromatography

  • M. Casas
  • L. A. Berrueta
  • B. Gallo
  • F. Vicente
Short Communications


A new method to predict the most suitable conditions for the solid phase extraction of 1,4-benzodiazepines and related compounds using C18 Sep-Pak carridges is proposed. The composition of the washing and elution solvents for the solid phase extraction of a test compound can be obtained from its capacity factor on a C18 HPLC column and an equation which relates capacity factors and solid phase extraction data of other similar compounds. The solid phase extraction data given in this paper can be used by others, there by saving considerable time and effort in the development of sample preparation methods. The suitability of the method was checked with two test compounds, showing good results.

Key Words

Column liquid chromatography Solid phase extraction 1,4-Benzodiazepines Drugs in plasma and urine 


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Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1992

Authors and Affiliations

  • M. Casas
    • 1
  • L. A. Berrueta
    • 1
  • B. Gallo
    • 1
  • F. Vicente
    • 1
  1. 1.Department of Analytical Chemistry, Faculty of SciencesUniversity of the Basque CountryBilbaoSpain

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