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Determination of the curcuminoid pigments in turmeric (Curcuma domestica Val) by reversed-phase high-performance liquid chromatography

Chromatographia volume 34, pages 73–77 (1992)Cite this article

Summary

A rapid, simple and reproducible reversed-phase high-performance liquid chromatographic method was developed for the quantitative determination of curcumin, demethoxycurcumin and bis-demethoxycurcumin in ethanolic extracts of turmeric. The pigments were separated on a styrene-divinylbenzene copolymer column (Hamilton PRP-1), using an acetonitrile-water (55∶45, v/v) mobile phase. The pigments were monitored with a diode-array detector at 425 nm. The limit of detection was 10.2 ng curcumin, 11.1 ng demethoxycurcumin and 6.2 ng bis-demethoxycurcumin. Comparison of HPLC and spectrophotometric results for the determination of the total curcuminoid content for a number of turmeric samples, reveal that the spectrophotometric method invariably yielded higher results, indicating an overestimation of curcuminoids.

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Authors and Affiliations

  1. Natural Resources Institute, Central Avenue, ME4 4TB, Chatham Maritime, Chatham, UK

    S. J. Taylor & I. J. McDowell

Authors
  1. S. J. Taylor
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  2. I. J. McDowell
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Taylor, S.J., McDowell, I.J. Determination of the curcuminoid pigments in turmeric (Curcuma domestica Val) by reversed-phase high-performance liquid chromatography. Chromatographia 34, 73–77 (1992). https://doi.org/10.1007/BF02290463

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  • DOI: https://doi.org/10.1007/BF02290463

Key Words

  • Column liquid chromatography
  • Curcuminoid pigments
  • Turmeric,Curcuma domestica Val