, Volume 34, Issue 1–2, pp 73–77 | Cite as

Determination of the curcuminoid pigments in turmeric (Curcuma domestica Val) by reversed-phase high-performance liquid chromatography

  • S. J. Taylor
  • I. J. McDowell


A rapid, simple and reproducible reversed-phase high-performance liquid chromatographic method was developed for the quantitative determination of curcumin, demethoxycurcumin and bis-demethoxycurcumin in ethanolic extracts of turmeric. The pigments were separated on a styrene-divinylbenzene copolymer column (Hamilton PRP-1), using an acetonitrile-water (55∶45, v/v) mobile phase. The pigments were monitored with a diode-array detector at 425 nm. The limit of detection was 10.2 ng curcumin, 11.1 ng demethoxycurcumin and 6.2 ng bis-demethoxycurcumin. Comparison of HPLC and spectrophotometric results for the determination of the total curcuminoid content for a number of turmeric samples, reveal that the spectrophotometric method invariably yielded higher results, indicating an overestimation of curcuminoids.

Key Words

Column liquid chromatography Curcuminoid pigments Turmeric,Curcuma domestica Val 


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  1. [1]
    K. R. Srinivasan, J. Pharm Pharmacol.5, 448 (1953).Google Scholar
  2. [2]
    J.W. Purseglove, E.G. Brown, C.L. Green, S.R.J. Robbins, Spices, Vol 2. Longman, Tropical Agriculture Series, 544 (1981).Google Scholar
  3. [3]
    International Standard Organization. Turmeric-Determination of Colouring Power-Spectrophotometric Method, 1982, ISO 5566.Google Scholar
  4. [4]
    American Spice Trade Association. ASTA Analytical Method 18.0- Colour Power of Turmeric.Google Scholar
  5. [5]
    A. Janssen, T. Gole, Chromatographia18, 546 (1984).Google Scholar
  6. [6]
    K. Jentszch, P. Spiegl, R. Kamitz, Sci. Pharm.38, 50 (1970).Google Scholar
  7. [7]
    H.H. Tonnesen, J. Karlsen, J. Chromatogr.259, 367 (1983).Google Scholar
  8. [8]
    R.M. Smith, B.A. Witowska, Analyst.109, 259 (1984).Google Scholar
  9. [9]
    P.J. Roughley, D.A. Whiting, JCS PerkinI, 2379 (1973).Google Scholar
  10. [10]
    R.G. Bailey, H.E. Nursten, I. McDowell, J. Chromatogr.542, 115 (1991).Google Scholar
  11. [11]
    M. Kuroyanagi, S. Natori, Yakugaku Zasshi.90, 1467 (1970).Google Scholar
  12. [12]
    U.-J. Saltzer, Flavours.206–10, 253 (1975).Google Scholar
  13. [13]
    M.N. Krishnamurthy, R. Padma Bai, C.P. Natarajan, S. Kuppuswamy, J. Food Sci. Technol. (India)12, 12 (1975).Google Scholar

Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1992

Authors and Affiliations

  • S. J. Taylor
    • 1
  • I. J. McDowell
    • 1
  1. 1.Natural Resources InstituteChatham MaritimeUK

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