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Chromatographia

, Volume 34, Issue 1–2, pp 51–55 | Cite as

Determination of tylosin and related macrolides in fermentation broth by gradient elution ion-pair liquid chromatography

  • F. L. Neely
Originals

Summary

A gradient elution ion-pair HPLC assay was developed for the analysis of tylosin and related macrolides in fermentation broth. The effect of the ion-pair agent concentration and identity was studied to optimize the reproducibility of the separation. The assay compares favorably with the biological assay and is sufficiently durable for the analysis of many samples per column.

Key Words

Ion-pair liquid chromatography Tylosin determination Fermentation 

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Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1992

Authors and Affiliations

  • F. L. Neely
    • 1
  1. 1.Lilly Research Laboratories, A Division of Eli Lilly and Co.IndianapolisUSA

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