, Volume 34, Issue 1–2, pp 19–24 | Cite as

Chiral separation of β-amino alcohols by capillary electrophoresis using cyclodextrins as buffer additives. I. Effect of varying operating parameters

  • K. D. Altria
  • D. M. Goodall
  • M. M. Rogan


Capillary electrophoresis has been used for the chiral analysis of two β-amino alcohol pharmaceutical compounds. Capillary zone electrophoresis conditions were used with β-cyclodextrin as a chiral mobile phase additive. The effects of variation of β-cyclodextrin concentration, temperature, pH, background electrolyte composition and concentration have been investigated. Optimum separations were achieved for clenbuterol using β-cyclodextrin at its solubility limit (16mM), the lowest practicable temperature (19°C), pH 4.0 and an electrolyte solution with a high ionic strength prepared from 0.1 M citric acid and 0.3 M Na2HPO4. For the development compound picumeterol and its (S)-enationmer, the optimum pH 4.0 buffer was prepared from 0.1 M citric acid and 0.2 M sodium acetate. Baseline separation with resolution greater than 2 was achieved for both compounds.

Key Words

Capillary electrophoresis Chiral resolution β-Cyclodextrin β-Amino alcohols Clenbuterol Picumeterol 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. [1]
    E. J. Areins, Clin. Pharmacol. Ther.42, 361 (1987).Google Scholar
  2. [2]
    D. W. Armstrong, W. Y. Liu, C. D. Chang, Anal. Chem.62, 914 (1990).Google Scholar
  3. [3]
    V. Schurig, M. Jung, in “Recent Advances in Chiral Separations”,D. Stevenson, I. D. Wilson, eds., Plenum Press, New York, 1990, p. 117.Google Scholar
  4. [4]
    A. Taylor, D. A. R. Williams, I. D. Wilson, J. Pharm. Biomed. Anal. in press (1991).Google Scholar
  5. [5]
    S. G. Allenmark, “Chromatographic Enantioseparation: Methods and Applications”, Ellis Horwood Ltd., Chichester, 1988.Google Scholar
  6. [6]
    W. J. Lough, ed., “Chiral Liquid Chromatography”, Blackie, Glasgow, 1989.Google Scholar
  7. [7]
    R. A. Wallingford, A. G. Ewing, Adv. Chromatogr.29, 1 (1989).Google Scholar
  8. [8]
    W. G. Kuhr, Anal. Chem.62, 403R (1990).Google Scholar
  9. [9]
    P. Gozel, E. Gassman, H. Michelsen, R. N. Zare, Anal. Chem.59, 44 (1987).Google Scholar
  10. [10]
    A. A. Cohen, A. Paulus, B. L. Karger, Chromatographia24, 15 (1987).Google Scholar
  11. [11]
    S. Terabe, M. Shibata, Y. Miyashita, J. Chromatogr.480, 403 (1989).Google Scholar
  12. [12]
    H. Nishi, T. Fukuyama, M. Matsao, S. Terabe, J. Chromatogr.515, 233 (1990).Google Scholar
  13. [13]
    S. Fanali, J. Chromatogr.474, 441 (1989).Google Scholar
  14. [14]
    S. Fanali, P. Bocek, Electrophoresis11, 757 (1990).Google Scholar
  15. [15]
    A. M. James, “Practical Physical Chemistry”, 2nd Edition, Churchill Ltd., London, 1964, p. 344.Google Scholar
  16. [16]
    D. Courtheyn, C. Desaever, R. Verhe, J. Chromatogr.564, 537 (1991).Google Scholar
  17. [17]
    C. Petterson, G. Schill, J. Chromatogr.204, 179 (1981).Google Scholar
  18. [18]
    L. Paduana, R. Sartorio, V. Vitagliano, L. Constantino, J. Solution Chem.19, 31 (1990).Google Scholar
  19. [19]
    F. Foret, P. Bocek, Adv. Electrophoresis3, 273 (1989).Google Scholar
  20. [20]
    D. Y. Pharr, Z. S. Fu, T. K. Smith, W. L. Hinze, Anal. Chem.61, 275 (1989).Google Scholar
  21. [21]
    H. Lauer, D. McManigill, Trends Anal. Chem.5, 11 (1986).Google Scholar
  22. [22]
    F. E. P. Mickers, F. M. Everaerts, T.P. E. M. Verheggen, J. Chromatogr.169, 1 and 11 (1979).Google Scholar
  23. [23]
    S. Terabe, Paper 248, Pittsburgh Conference, Chicago, March 4–8, 1991.Google Scholar
  24. [24]
    E. Bergstrom, D. M. Goodall, M. Survay, S. J. Williams, manuscript in preparation, 1991.Google Scholar
  25. [25]
    R. C. Weast, ed., “CRC Handbook of Chemistry and Physics”, CRC Press Inc., Boca Raton, 1982–83, Table F-40.Google Scholar

Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1992

Authors and Affiliations

  • K. D. Altria
    • 1
  • D. M. Goodall
    • 2
  • M. M. Rogan
    • 1
  1. 1.Department of Pharmaceutical AnalysisGlaxo Group Research Ltd.WareUK
  2. 2.Department of ChemistryUniversity of YorkYorkUK

Personalised recommendations