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A practical description of retention in reversed phase chromatography using four parameters

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Summary

Retention of small molecules on reversed phase or other hydrophobic stationary phases can be represented by k′ values of members of a homologous series at varying solvent compositions. The evaluation of simple linear relationships leads to the introduction of a set of four parameters that contain all the information about retention behaviour of this homologous series in the binary solvent system chosen. It is possible to extrapolate to a hypothetical non-hydrophobic molecule and a solvent, the polarity of which equals that of the stationary phase, to characterize the properties of the stationary phase — eluent system. In addition, a chromatographic method for determination of the phase ratio of the column is proposed.

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Author notes

  1. M. Czok

    Present address: Division of Analytical Chemistry, ORNL, Oakridge, Tennessee, USA

Authors and Affiliations

  1. Angewandte Physikalische Chemie, Universität des Saarlandes, D-6600, Saarbrücken, FRG

    M. Czok & H. Engelhardt

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  1. M. Czok
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  2. H. Engelhardt
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Czok, M., Engelhardt, H. A practical description of retention in reversed phase chromatography using four parameters. Chromatographia 27, 5–14 (1989). https://doi.org/10.1007/BF02290396

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  • DOI: https://doi.org/10.1007/BF02290396

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