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A general method for the quantitative determination of catecholamines in body fluids using off-line sample pretreatment and HPLC-ED analysis

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Summary

Reversed phase HPLC with electrochemical detection (HPLC-ED) was used for quantitative determination of adrenaline, noradrenaline, and dopamine in several complex biological matrices, including plasma, uremic plasma, and urine. Three different methods of sample preparation for use in this clinical chemistry were tested. These were adsorption of catecholamines on alumina, organic solvent extraction after complex formation with diphenylborate, and adsorption of catecholamines on a cation exchange gel followed by organic solvent extraction of the elute. The selectivity and precision of the three methods were evaluated. The organic solvent extraction proved to be more precise and selective than adsorption on alumina (adrenaline: cv=3.80% vs. 7.58%; noradrenaline: cv=1.70% vs. 4.26%); it also proved suitable for use in the routine quantitative determination of catecholamines in plasma from patients with normal renal function (creatinine <1.2 mg/dl). However when working with uremic plasma or urine, a more selective sample preparation was required. In this case the adsorption of catecholamines on a cation exchange gel followed by organic solvent extraction of the elute was sufficiently selective and precise and thus allowed a reliable quantitative determination of adrenaline and noradrenaline from rather complex biological matrices (adrenaline: cv=6.2%; noradrenaline: cv=2.8%). Use of this specific method showed that basal plasma catecholamine levels in dialysis patients are comparable to those found in patients with normal renal function (adrenaline: 47.7±22.2 pg/ml; noradrenaline: 310.3±121.4 pg/ml).

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References

  1. P. Hjemdahl, Am. J. Physiol.247, 13–20 (1984).

    Google Scholar 

  2. H.-J. Bauch, J. Grünwald, P. Vischer, U. Gerlach, W. H. Hauss, Exp. Pathol.31, 193–204 (1987).

    Google Scholar 

  3. H.-J. Bauch, U. Kelsch, K.-H. Grotemeyer, R. Buchwalsky, W. H. Hauss, in: Frühveränderungen bei der Atherogenese, W. Zuckerschwerdt-Verlag, München (1987), 147–155.

    Google Scholar 

  4. H.-J. Bauch, H. Schulte, E. Selhorst, U. Kelsch, R. Buchwalsky, W. H. Hauss, in: Die Anwendung aktueller Methoden in der Arterioskleroseforschung, (1988) im Druck.

  5. A. D. Falconer, D. Lake, I. A. Macdonald, J. Neurosci. Meth.6, 261–171 (1982).

    Google Scholar 

  6. H.-J. Bauch, U. Kelsch, W. H. Hauss, J. Clin. Chem. Clin. Biochem.24, 651–658 (1986).

    Google Scholar 

  7. T. A. Durkin, E. J. Caliguri, I. N. Mefford, D. M. Lake, I. A. Macdonald, E. Sundstrom, E. Jonsson, Life Sci.37, 1803–1810 (1985).

    Google Scholar 

  8. B. Dirks, C. Verwalter, A. Grünert, F. W. Ahnefeld, Chromatographia25, 223–229 (1988).

    Google Scholar 

  9. A. M. Krstulovic, J. Chromatogr.229, 1–34 (1982).

    Google Scholar 

  10. W. Bauersfeld, D. Ratge, E. Knoll, H. Wisser Fresenius Z. Anal. Chem.317, 679–680 (1984).

    Google Scholar 

  11. F. Smedes, J. C. Kraak, H. Poppe, J. Chromatogr.231, 25–39 (1982).

    Google Scholar 

  12. I. A. Macdonald, D. M. Lake, J. Neurosci. Meth.13, 239–248 (1985).

    Google Scholar 

  13. A. H. Anton, D. F. Sayre, J. Pharmacol. Exp. Ther.138, 360–374 (1962).

    Google Scholar 

  14. World Health Organization, Demographic Year Book, Genf 1978.

  15. H.-J. Bauch, W. H. Hauss, Chromatographia,28, 69 (1989).

    Google Scholar 

  16. D. Ratge, R. Augustin, H. Wisser, Int. J. Artif. Organs.6, 255–260 (1983).

    Google Scholar 

  17. C. R. Lake, M. G. Ziegler, M. D. Coleman, I. J. Kopin, Cardiovasc. Med. 1099–1111 (1979).

  18. C. N. Corda, J. Sharma, R. H. McDonald, Nephron25, 267–272 (1980).

    Google Scholar 

  19. R. Darwish, A. N. Elias, N. D. Vaziri, M. Pahl, D. Powers, J. Stokes, Arch. Intern. Med.144, 69–71 (1984).

    Google Scholar 

  20. A. N. Elias, N. D. Vaziri, M. Maksy, Arch. Intern. Med.145, 1013–1015 (1985).

    Google Scholar 

  21. N. O. Atuk, C. J. Bailey, S. Turner, M. J. Peach, F. B. Westervelt, Trans. Am. Soc. Artif. Int. Organs.22, 195–200 (1976).

    Google Scholar 

  22. B. P. McGrath, J. G. Ledingham, C. R. Benedict, Clin. Sci. Mol. Med.55, 89–96 (1978).

    Google Scholar 

  23. E. L. Bravo, R. C. Tarazi, R. W. Gifford, B. H. Stewart, N. Engl. J. Med.,301, 682–686 (1979).

    Google Scholar 

  24. P. Hjemdahl, Am. J. Physiol.247, E13-E20 (1984).

    Google Scholar 

  25. J. N. Cohn, T. B. Levine, M. T. Olivary, V. Garberg, D. Lura, G. S. Francis, N. Engl. J. Med.311, 682–686 (1984).

    Google Scholar 

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Authors and Affiliations

  1. Institut für Arterioskleroseforschung an der Universität Münster, Domagkstr. 3, D-4400, Münster, FRG

    H. -J. Bauch & E. Strüwer

  2. Labor Dr. B. Eveld, Nienkampstr. 1, D-4300, Essen, FRG

    U. Kelsch

Authors
  1. H. -J. Bauch
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  2. E. Strüwer
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  3. U. Kelsch
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Bauch, H.J., Strüwer, E. & Kelsch, U. A general method for the quantitative determination of catecholamines in body fluids using off-line sample pretreatment and HPLC-ED analysis. Chromatographia 28, 78–84 (1989). https://doi.org/10.1007/BF02290387

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  • DOI: https://doi.org/10.1007/BF02290387

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