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Nucleotide and aldehyde analysis by HPLC for determination of radical induced damage

  • Würzburg Chromatography Colloquium
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Summary

The analysis of two metabolite groups, nucleotides and aldehydes, is necessary for assessment of oxygen radical metabolism during hypoxia and reoxygenation.

Nucleotides and their derivatives were determined by HPLC using gradient elution with 10 mM NH4H2PO4 buffer containing 2 mM t-butylammoniumphosphate and acetonitrile.

Aldehydes occuring after lipid peroxidation were analyzed by derivatisation to dinitrophenylhydrazones followed by TLC and HPLC separation with methanol/water on an ODS column.

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References

  1. D. A. Hems, J. T. Brosnan Biochem. J.120, 105 (1970).

    Google Scholar 

  2. S. Marubayashi, M. Takenaka, K. Dohi, H. Ezaki, T. Kawasaki, Transplantation30, 294 (1980).

    Google Scholar 

  3. M.-F. Vincent, G. van den Berghe, H. C. Hers, Biochem. J.202, 117 (1982).

    Google Scholar 

  4. D. N. Granger, G. Rutili, J. M. McCord, Gastroenterology81, 22 (1981).

    Google Scholar 

  5. J. M. McCord, R. S. Roy, Can. J. Physiol. Pharmacol.60, 1346 (1982).

    Google Scholar 

  6. H. Osswald, Pflügers Archiv371, 45 (1977).

    Google Scholar 

  7. A. Benedetti, M. Comporti, Bioelectrochemistry and Bioenergetics18, 187 (1987).

    Google Scholar 

  8. E. Schauenstein, G. Schatz, G. Benedikt, Monatsh. Chem.92, 442 (1961).

    Google Scholar 

  9. E. Schauenstein, H. Esterbauer, Monatsh. Chem.94, 164 (1963).

    Google Scholar 

  10. D. W. Allen, J. J. Cierzan, J. Lab. Clin. Med.112, 87 (1988).

    Google Scholar 

  11. M. N. Berry, D. S. Friend, J. of Cell Biol.43, 506 (1969).

    Google Scholar 

  12. G. van den Berghe, F. Bontemps, H. G. Hers, Biochem. J.188, 913 (1980).

    Google Scholar 

  13. A. Werner, W. Siems, H. Schmidt, I. Rapoport, G. Gerber, R. T. Toguzov, Y. V. Tikhonov, A. M. Pimenov, J. Chromatogr., Biomed. Appl.421, 257 (1987).

    Google Scholar 

  14. H. Esterbauer, K. H. Cheeseman, M. U. Dianzani, G. Poli, T. F. Slater, Biochem. J.208, 129 (1982).

    Google Scholar 

  15. G. Poli, M. U. Dianzani, K. H. Cheeseman, T. F. Slater, J. Lang, H. Esterbauer, Biochem. J.227, 629 (1985).

    Google Scholar 

  16. D. R. Webster, G. D. Boston, D. M. Paton, J. Liquid Chromatogra.8, 603 (1985).

    Google Scholar 

  17. V. Stocchi, L. Cucchiarini, F. Canestrari, M. P. Piacentini, G. Fornaini, Anal. Biochem.167, 181 (1987).

    Google Scholar 

  18. D. Perrett, in: HPLC of small molecules — a practical approach,C. K. Lim (Ed.), IRL Press, Oxford, 1986, p. 221.

    Google Scholar 

  19. H. Esterbauer, in: Free Radicals, Lipid Peroxidation and Cancer,D. C. H. McBrien andT. F. Slater (Eds.), Academic Press, London, 1982, p. 101.

    Google Scholar 

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Authors and Affiliations

  1. Institute of Biochemistry, Humboldt-University, Hessische Straße 3/4, 1040, Berlin, GDR

    A. Werner, T. Grune, W. Siems, W. Schneider & G. Gerber

  2. Department of Biochemistry, Tokyo University, School of Medicine, 11-1 Kaga, 2 Chome, Itabashi-Ku, 173, Tokyo, Japan

    H. Shimasaki

  3. Institute of Biochemistry, University of Graz, Schubertstr. 1, A-8010, Graz, Austria

    H. Esterbauer

Authors
  1. A. Werner
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  2. T. Grune
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  3. W. Siems
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  4. W. Schneider
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  5. H. Shimasaki
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  6. H. Esterbauer
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  7. G. Gerber
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Werner, A., Grune, T., Siems, W. et al. Nucleotide and aldehyde analysis by HPLC for determination of radical induced damage. Chromatographia 28, 65–68 (1989). https://doi.org/10.1007/BF02290385

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  • DOI: https://doi.org/10.1007/BF02290385

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