, Volume 28, Issue 1–2, pp 59–64 | Cite as

Isolation and identification of sialylcompounds from hemofiltrate

  • H. Brunner
  • G. Weißhaar
  • H. Friebolin
  • H. Mann
  • W. Baumann
  • H. -G. Sieberth
Würzburg Chromatography Colloquium


Hemofiltrate of patients with end stage renal disease is a source of sialyl compounds of unusual composition which accumulate in uremia. The large volumes involved (20–30l), are concentrated and desalted by modified reverse osmosis using membranes with a nominal cut-off of 500 D. The transport of the individual solutes during this process is monitored by reversed-phase HPLC of the permeates and retentates, respectively. By a combination of size-exclusion and ion-exchange chromatography 11 sialyl compounds are isolated preparatively. All structures have been determined using one and two dimensional NMR spectroscopy, enzymatic degradation and FAB mass spectrometry.

Key Words

Sialyl compounds Uremic toxins Hemofiltration Renal failure NMR-spectroscopy 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. [1]
    H. Brunner, H. Mann, Contr. Nephrol., vol.44, pp. 14–39 (Karger, Basel 1985).Google Scholar
  2. [2]
    H. Brunner, H. Mann, U. Essers, R. Schultheis, T. Byrne, R. Heintz, Artif. Organs2, 375–377 (1978).Google Scholar
  3. [3]
    H. Brunner, H. Mann J. Chromatogr.297, 405–416 (1984).Google Scholar
  4. [4]
    G. Weißhaar, Sialinsäurehaltige Verbindungen im Hämofiltrat chronisch urämischer Patienten: Isolierung und Strukturaufklärung. Dissertation, Universität Heidelberg (1987).Google Scholar
  5. [5]
    G. Weißhaar, W. Baumann, H. Friebolin, H. Brunner, H. Mann, H.-G. Sieberth, H.-J. Opferkuch, Biol. Chem. Hoppe-Seyler368, 647–658 (1987).Google Scholar
  6. [6]
    G. Weißhaar, W. Baumann, H. Friebolin, H. Brunner, H. Mann, H.-G. Sieberth, H.-J. Opferkuch, Biol. Chem. Hoppe-Seyler368, 1545–1558 (1987).Google Scholar
  7. [7]
    L. Warren, J. Biol. Chem.234, 1971–1975 (1959).Google Scholar
  8. [8]
    R. Kuhn, R. Brossmer, Chem. Berichte89, 2013–2025 (1956).Google Scholar
  9. [9]
    J. K. Huttunen, Ann. Med. Exp. Biol. Fenn.44 (Suppl. 12), 1–60 (1966).Google Scholar
  10. [10]
    J. Parkinnen, J. Finne, Eur. J. Biochem.136, 355–361 (1983).Google Scholar
  11. [11]
    G. Le Moel, G. Strecker, S. Troupel M. Dolegeal, C. Jacobs, A. Galli, J. Agneray, Artif. Organs4 (Suppl.), 37–40 (1980).Google Scholar
  12. [12]
    R. Kuhn, A. Gauhe, Chem. Ber.98, 395–413 (1965).Google Scholar
  13. [13]
    E. Berman, Carbohydr. Res.118, 9–20 (1983).Google Scholar
  14. [14]
    P. Maury, Clin. Chim. Acta71, 335–338 (1976).Google Scholar
  15. [15]
    J. Parkinnen, J. Finne, Eur. J. Biochem.140, 427–431 (1984).Google Scholar
  16. [16]
    J. Finne, T. Krusius, H. Rauvala, Biochem. Biophys. Res. Comm.74, 405–410 (1977).Google Scholar
  17. [17]
    J. Finne, T. Krusius, H. Rauvala, K. Hemminki, Eur. J. Biochem.77, 319–323 (1977).Google Scholar
  18. [18]
    M. L. Hayes, F. J. Castellino, J. Biol. Chem.254, 8768–8780 (1979).Google Scholar
  19. [19]
    Y. Endo, Y. Tachibana, S. Tojo, A. Kobata, J. Biochem.85, 669–679 (1979).Google Scholar
  20. [20]
    M. J. Kessler, M. S. Reddy, R. H. Shah, O. P. Bahl, J Biol. Chem.254, 7901–7908 (1979).Google Scholar
  21. [21]
    M. J. Kessler, T. Mise, R. D. Ghai, O. P. Bahl, J. Biol. Chem.254, 7909–7914 (1979).Google Scholar
  22. [22]
    V. T. Marchesi, H. Furthmayr, M. Tomita Ann. Rev. Biochem.45, 667–698 (1976).Google Scholar
  23. [23]
    M. Tomita, V. T. Marchesi, Proc. Natl. Acad. Sci. USA72, 2964–2968 (1975).Google Scholar
  24. [24]
    R. Prohaska, T. A. W. Koerner Jr., J. M. Armitage, F. Furthmayr, J. Biol. Chem.256, 5781–5791 (1981).Google Scholar
  25. [25]
    M. Lemmonnier, R. Bourillon, Biochem. Biophys. Res. Comm.64, 226–231 (1975).Google Scholar
  26. [26]
    J. W. Eschbach, J. W. Adamson, Kidney Int.28, 1–5 (1985).Google Scholar
  27. [27]
    A. B. Shaw, Brit. Med. J.2, 213–215 (1967).Google Scholar
  28. [28]
    A. R. Poole, Biochem. J.236, 1–14 (1986).Google Scholar

Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1989

Authors and Affiliations

  • H. Brunner
    • 1
  • G. Weißhaar
    • 2
  • H. Friebolin
    • 2
  • H. Mann
    • 1
  • W. Baumann
    • 2
  • H. -G. Sieberth
    • 1
  1. 1.Innere Medizin IITechnische Hochschule AachenAachenFRG
  2. 2.Organisch-Chemisches Institut der Universität HeidelbergFRG

Personalised recommendations