Summary
Pre-column derivatization followed by reversed-phase HPLC has become widely used as a sensitive and speedy method of amino acid analysis.
The technique has been improved by the development of simple and reproducible strategies for derivatization of the amino acids with DABS and for the HPLC analysis. Results obtained indicate that the method has a high sensitivity is well and suited to generate precise amino acid composition information from peptides and proteins.
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Schneider, H.J. Amino acid analysis using DABS-CL. Chromatographia 28, 45–48 (1989). https://doi.org/10.1007/BF02290382
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DOI: https://doi.org/10.1007/BF02290382