Summary
A computer-assisted method is presented for the optimization of separation in gradient elution reversed-phase HPLC. The method is based on a polynomial estimation from nine preliminary experiments according to a two-factor (initial solvent composition C and gradient time T) rectangular design. This is followed by a two-dimension computer scanning technique. Resolution is used as the selection criterion. Good agreement was obtained between predicted data and experimental results.
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References
M. W. Watson, P. W. Carr, Anal. Chem51, 1835 (1979).
F. Dondi, Y. D. Kahie, G. Lodi, P. Reschiglian, C. Pietrogrande, C. Bighi, G. P. Cartoni, Chromatographia23, 844 (1987).
T. H. Jupille, J. W. Dolan, L. R. Snyder, Am. Lab.20, 20 (1988).
B. F. D. Christ, B. S. Cooperman, L. R. Snyder, J. Chromatogr.459, 1 (1988).
B. F. D. Christ, L. R. Snyder, J. Chromatogr.458, 25 (1988).
B. F. D. Christ, L. R. Snyder J. Chromatogr.458, 43 (1988).
Y. Baba, M. Fukuda, N. Yoza, J. Chromatogr.458, 385 (1988).
Y. Baba, Minekok. Ito, J. Chromatogr.485, 647 (1989).
P. Jandera, J. Chromatogr.485, 113 (1989).
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Wang, Q.S., Gao, R.Y., Yan, B.W. et al. Statistical scanning method for the optimization of gradient elution high performance liquid chromatography. Chromatographia 38, 187–190 (1994). https://doi.org/10.1007/BF02290335
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DOI: https://doi.org/10.1007/BF02290335