Summary
A high-performance liquid chromatographic method for the simultaneous determination of bidesmosidic and monodesmosidic saponins from the leaves ofHedera helix L. using a light-scattering detector is proposed.
A satisfactory separation of the bidesmosidic and monodesmosidic saponins is obtained in 25 min on a reversed phase RP18 using an acetonitrile-water mixture as eluant. Hederasaponin C and α-hederin were found to be the major saponins of the leaves. The linearity of response, repeatability and reproducibility of the proposed method were tested. The detection limits for hederasaponin C and α-hederin were 0,1 and 0.05 μg/20 μl respectively.
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Crespin, F., Calmes, M., Elias, R. et al. High-performance liquid chromatographic determination of saponins fromHedera helix L. using a light-scattering detector. Chromatographia 38, 183–186 (1994). https://doi.org/10.1007/BF02290334
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DOI: https://doi.org/10.1007/BF02290334