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Effects of (D-Ala6, Pro9N ethylamide) — LHRH on plasma levels of gonadotropin, 17α,20β-dihydroxy-4-pregnen-3-one and testosterone in male goldeye (hiodon alosoides Rafineque)

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Abstract

Male goldeye were treated with (D-Ala6, Pro9-N ethylamide) — LHRH (LHRH-A) in saline or a silastic pellet (100 µg.kg−1 body weight) and changes in plasma gonadotropin (GtH), 17α,20β-dihydroxy-4-pregnen-3-one (17α,20βP) and testosterone (T) levels were determined by radioimmunoassay. LHRH-A increased plasma GtH levels, with the response to LHRH-A in saline being of much greater magnitude and duration than the response to silastic pellet implants. Seasonal differences were found in the response to LHRH-A. Spermiated fish were the most responsive, recrudescing fish the least, and regressed fish displayed an intermediate response. Plasma 17α,20βP levels were elevated in response to LHRHA in fish of all sexual stages although the magnitude of the increase was not related to the magnitude of the increase in GtH levels. Treatment with LHRH-A also resulted in a transitory increase in plasma T levels. The endocrine control of GtH and steroid secretion in goldeye is discussed in relation to studies in cyprinids and salmonids.

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Author notes

  1. N. W. Pankhurst

    Present address: Fisheries Research Centre, P.O. Box 297, Wellington, New Zealand

Authors and Affiliations

  1. Department of Zoology, University of Alberta, T6G 2E9, Edmonton, Alberta, Canada

    N. W. Pankhurst, G. van der Kraak & R. E. Peter

  2. Laboratoire de Physiologie des Poissons, INRA, Campus de Beaulieu, 35042, Rennes cédex, France

    B. Breton

Authors
  1. N. W. Pankhurst
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  2. G. van der Kraak
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  3. R. E. Peter
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  4. B. Breton
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Address for reprint requests: Department of Zoology, University of Alberta, Edmonton, Alberta T6G 2E9 Canada.

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Pankhurst, N.W., van der Kraak, G., Peter, R.E. et al. Effects of (D-Ala6, Pro9N ethylamide) — LHRH on plasma levels of gonadotropin, 17α,20β-dihydroxy-4-pregnen-3-one and testosterone in male goldeye (hiodon alosoides Rafineque). Fish Physiol Biochem 1, 163–170 (1986). https://doi.org/10.1007/BF02290257

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