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Urinary organ specific neoantigen

A potentially diagnostic test for colorectal cancer

  • Gastrointestinal Oncology
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Abstract

Urinary organ-specific neoantigen from colorectal cancer patients has been used to make a monoclonal antibody, BAC 18.1. In this study we assessed the potential of this antibody for the diagnosis of colorectal cancer. We evaluated binding in both urine and effluent samples and compared it with effluent carcinoembryonic antigen standardized for both volume (nanograms per milliliter) and protein. Urinary organ-specific antigen as detected by BAC 18.1 was significantly greater in 29 cancer patients (A405:0.717±0.500) vs 27 controls [0.121 ±0.273 (P<0.05)]. Considerable overlap of binding of BAC 18.1 was observed in the colonic effluent of patients with CRC (N=13), adenomas (N=26), inflammatory bowel disease (N=8), or having a normal colonoscopic examination (N=24). CEA levels (nanograms per milliliter) were significantly elevated in the effluent samples of patients with a past history of colorectal cancer, as compared to that of normal individuals (P<0.05). The presence of the Mr 30,000 organ-specific neoantigen in colonic effluent was also demonstrated by western blot. Organ-specific neoantigen originates in the colon and is excreted into the urine, so the BAC 18.1 binding levels in the urine may be a diagnostic aid for CRC.

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References

  1. Sidransky D, Tokino T, Hamilton SR, Kinzler KW, Levin B, Frost P, Vogelstein B: Identification of ras oncogene mutations in the stool of patients with curable colorectal tumors. Science 256:102–105, 1992

    Google Scholar 

  2. Darmon E, Tobi M, Rozen P: Newer tests, on the colonic effluent, for identifying persons with colorectal neoplasia.In Advances in Large Bowel Cancer: Policy, Prevention, Research and Treatment, Vol 18. Frontiers of Gastrointestinal Research. P Rozen, CB Reich, SJ Winawer (eds), Basel, Karger, 1991, pp 236–247

    Google Scholar 

  3. Gaspari MM, Brennan PT, Solomon SM, Elson CG: A method of obtaining, processing, and analyzing human intestinal secretions for antibody content. J Immunol Methods 100:85–91, 1988

    Google Scholar 

  4. D'Mahony S, Barton JR, Crichton S, Ferguson A: Appraisal of gut lavage in the study of intestinal humoral immunity. Gut 31:1341–1344, 1990

    Google Scholar 

  5. Tobi M, Steinberg W, O'Kieffe D, Trujillo N, Nochomovitz LE, Steinberg WM: Detection of carcinoembryonic antigen in colonic effluent by specific anti-CEA monoclonal antibodies. Cancer Lett 67:47–54, 1992

    Google Scholar 

  6. Tobi M, Maliakkal BJ, Alousi MA, Voruganti V, Shafiuddin M, Yang S, Gesell MS, An TS, Hatfield JS, Fligiel SEM, Kaila V, Goo RH, Luk GD: Cellular distribution of colonic adenoma associated antigen as defined by monoclonal antibody Adnab-9. Scand J Gastroenterol 27:737–742, 1992

    Google Scholar 

  7. Nutter RL, Kettering JD: Leucocyte adherence inhibition: history, biological basis and clinical usefulness. Eur J Cancer Clin Oncol 23:1257–1260, 1987

    Google Scholar 

  8. Shani A, Rozen P, Fink A, Bass D, Fraser D, Bentwich Z: Limited value of leucocyte adherence inhibition in screening for colorectal neoplasia in high-risk groups. Cancer Prev Detect 13:311–316, 1989

    Google Scholar 

  9. Fink A, Harpaz N, Weizman Z, Shani A, Bentwich Z: The isolation of colon cancer organ specific neoantigen by the use of leucocyte adherence inhibition assay and monoclonal antibodies. Anticancer Res 6:813–818, 1986

    Google Scholar 

  10. Freed DLG, Taylor G: Carcinoembryonic antigen in faeces. Br Med J 1:85–87, 1972

    Google Scholar 

  11. Elias EG, Holyoke ED, Chu TM: Carcinoembryonic antigen in feces and plasma of normal subjects and patients with colorectal carcinoma. Dis Colon Rectum 17:38–41, 1974

    Google Scholar 

  12. Stubbs RS, Nadkami DM, Monsey HA: Fecal carcinoembryonic antigen in colorectal cancer patients. Gut 27:901–905, 1986

    Google Scholar 

  13. Fujimoto S, Kitsukawa Y, Itoh K: Carcinoembryonic antigen (CEA) in gastric juice or feces as an aid in the diagnosis of gastrointestinal cancer. Ann Surg 189:34–38, 1979

    Google Scholar 

  14. Winawer SJ, Fleischer M, Green S, Bhargava D, Leidner SD, Boyle C, Sherlock P, Schwartz MK: Carcinoembryonic antigen in colonic lavage. Gastroenterology 92:719–722, 1977

    Google Scholar 

  15. Vellacott KD, Groom D, Balfour TW, Baldwin RW, Hardcastle JD: Tumor associated products in colonic lavage fluid. Clin Oncol 8:61–67, 1982

    Google Scholar 

  16. Tobi M, Darmon E, Phillips T, Heller T, Rozen P, Nochomovitz LE, Steinberg WM: Increased expression of a putative adenoma associated antigen in precolonoscopic effluent of patients with colorectal cancer. Cancer Lett 50:21–25, 1990

    Google Scholar 

  17. Pöyhönen MJ, Uusitala UM, Kari A, Takala JA, Alakuijala LA, Eloranta TO: Urinary polyamines: Importance of circadian rhythm, age, sex, menstrual cycle, weight, and creatinine excretion. Am J Clin Nutr 52:746–751, 1990

    Google Scholar 

  18. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ: Protein measurements with Folin phenol reagent. J Biol Chem 193:265–275, 1951

    Google Scholar 

  19. DiPalma JA, Brady CE, Steward DL, Karlin DA, McKinney MK, Clement DJ, Coleman TW, Pierson WP: Comparison of colon cleansing methods in preparation for colonoscopy. Gastroenterology 86:856–860, 1964

    Google Scholar 

  20. Goa J: A microbiuret method for protein determination. Scand J Clin Lab Invest 5:218–222, 1953

    Google Scholar 

  21. Tobi M, Darmon E, Konikoff F, Stadler Y, Rozen P: Should we let sleeping pegs lie? Gastroenterology 95:1693–1694, 1988

    Google Scholar 

  22. Tobi M, Darmon E, Rozen P, Epstein N, Konikoff F, Stadler Y, Harpaz N, Fink AK, Bentwich Z, Maliakkal B, Luk GD: Oral colon lavage preparations may interfere with ELISA detection of tumor associated antigens in colonic effluent. Dig Dis Sci 36:1448–1452, 1991

    Google Scholar 

  23. Towbin H, Staelin T, Gordon J: Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedures and some applications. Proc Natl Acad Sci USA 76:4350–4354, 1979

    Google Scholar 

  24. Laemmli VK: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685, 1970

    Google Scholar 

  25. Ingram D, Bloch R (eds.): Mathematical Models in Medicine, Pt 1. New York, John Wiley & Sons, 1984

    Google Scholar 

  26. Fletcher RH: Carcinoembryonic Antigen. Ann Intern Med 104:66–73, 1986

    Google Scholar 

  27. Tobi M, Chintalapani S, Kaila V, Halline A, Fink A, Bentwich Z: Diagnosis of colorectal cancer (CRC) by monoclonal antibodies (MAbs) tailored for detection bodily fluids. Proc Am Assoc Cancer Res 33:96, 1992

    Google Scholar 

  28. Wagener C, Muller-Wallraf R, Nisson S, Groner J, Breuer H: Localization and concentration of carcinoembryonic antigen (CEA) in gastrointestinal tumors: Correlation with CEA levels in the plasma. J Natl Cancer Inst 67:539–547, 1981

    Google Scholar 

  29. Tobi M, Maliakkal B, Voruganti V, Alousi M, Yang S, Goo B, Reddy J, Luk GD: The use of Adnab-9, A monoclonal antibody raised against colonic adenoma antigens, in the diagnosis of colorectal neoplasia. Gastroenterology 100:A406, 1991

    Google Scholar 

  30. Tobi M, Darmon E, Rozen P, Avigdor S, Rattan J, Santo M, Barnea E: Rectal mucosal biotransformation in subjects at risk for colorectal cancer. Scand J Gastroenterol 28:958–962, 1993

    Google Scholar 

  31. Rozen P, Tobi M, Darmon E, Kaufman L: Colonic cytology: A simplified method of collection and initial results. Acta Cytol 34:627–631, 1990

    Google Scholar 

  32. Tobi M, Elitsur Y, Moyer MP, Halline A, Deutsch M, Nochomovitz L, Luk GD: Mucosal origin and shedding of an early colonic tumor marker defined by Adnab-9 monoclonal antibody. Scand J Gastroenterol 28:1025–1034, 1993

    Google Scholar 

  33. Tobi M, Feng-Chi L, Ronai Z: Detection ofK-ras mutation in colonic effluent samples from patients without evidence for colorectal carcinoma. J Natl Cancer Inst 86:1007–1010, 1994

    Google Scholar 

  34. Frommer DJ, Kapparis A, Brown MK: Improved screening for colorectal cancer by immunological detection of occult blood. Br Med J 296:1092–1094, 1988

    Google Scholar 

  35. Nakayama T, Yasuoka H, Kishino T, Ohguchi H, Tadaka M: ELISA for occult faecal albumin. Lancet 1:1368–1369, 1987

    Google Scholar 

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Authors and Affiliations

  1. Department of Gastroenterology, Tel Aviv Medical Center, 6 Weizman St, Tel Aviv, Israel

    Martin Tobi MB, ChB, Elizabeth Darmon MSc, Paul Rozen MBBS, Nurit Harpaz MSc, Aron Fink MD, PhD, Benedict Maliakkal MD, Allan Halline MD, Sohrab Mobarhan MD & Zvi Bentwich MD

  2. The Ben-Ari Immunological Institute, Kaplan Hospital, Rehovot, Israel

    Martin Tobi MB, ChB, Elizabeth Darmon MSc, Paul Rozen MBBS, Nurit Harpaz MSc, Aron Fink MD, PhD, Benedict Maliakkal MD, Allan Halline MD, Sohrab Mobarhan MD & Zvi Bentwich MD

  3. Division of Gastroenterology, Department of Medicine, Wayne State University Medical School, Harper Hospital, 3990 John R St., 48201, Detroit, Michigan

    Martin Tobi MB, ChB, Elizabeth Darmon MSc, Paul Rozen MBBS, Nurit Harpaz MSc, Aron Fink MD, PhD, Benedict Maliakkal MD, Allan Halline MD, Sohrab Mobarhan MD & Zvi Bentwich MD

  4. Division of Gastroenterology, University of Illinois at Chicago, Chicago, Illinois

    Martin Tobi MB, ChB, Elizabeth Darmon MSc, Paul Rozen MBBS, Nurit Harpaz MSc, Aron Fink MD, PhD, Benedict Maliakkal MD, Allan Halline MD, Sohrab Mobarhan MD & Zvi Bentwich MD

  5. Loyola University Medical Center, Maywood, Illinois

    Martin Tobi MB, ChB, Elizabeth Darmon MSc, Paul Rozen MBBS, Nurit Harpaz MSc, Aron Fink MD, PhD, Benedict Maliakkal MD, Allan Halline MD, Sohrab Mobarhan MD & Zvi Bentwich MD

Authors
  1. Martin Tobi MB, ChB
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  2. Elizabeth Darmon MSc
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  3. Paul Rozen MBBS
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  4. Nurit Harpaz MSc
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  5. Aron Fink MD, PhD
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  6. Benedict Maliakkal MD
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  7. Allan Halline MD
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  8. Sohrab Mobarhan MD
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  9. Zvi Bentwich MD
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Additional information

The work reported in this paper was supported in part by a grant from the Israeli Cancer Association and Tel Aviv University, and in part by grants from the Israel Cancer Association and the Sackler School of Medicine, Tel Aviv, Israel.

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Tobi, M., Darmon, E., Rozen, P. et al. Urinary organ specific neoantigen. Digest Dis Sci 40, 1531–1537 (1995). https://doi.org/10.1007/BF02285204

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  • DOI: https://doi.org/10.1007/BF02285204

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