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Transferrin is not involved in initial uptake process of iron in rat duodenal mucosa

Ultrastructural study by X-ray energy spectrometry

  • Intestinal Disorders, Inflammatory Bowel Disease, Immunology, and Microbiology
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Abstract

The role of transferrin in iron absorption by the duodenal mucosa in rats with iron deficiency and controls was evaluated immunohistochemically. Ferric iron was administered to each rat using a metallic gastric tube. Transferrin was stained by an immunoperoxidase method and iron with Prussian blue in the same duodenal sections and observed by light microscopy. The localization of transferrin differed from that of ferric iron both in rats with iron deficiency and in controls. In iron-deficient rats, transferrin was weakly stained after iron administration but was strongly stained after saline administration. In contrast, in controls, transferrin was weakly stained after saline administration but was strongly stained after iron administration. By electron microscopy, x-ray energy spectrometric analysis of the transferrin-positive areas showed no iron peak. In iron-deficient rats, accumulation of electron-dense transferrinnegative microgranules was observed in some of the duodenal columnar epithelium. X-ray energy spectrometric analysis of this area revealed iron, indicating iron absorption. These results suggest that mucosal transferrin does not act as a shuttle protein in iron absorption via the rat duodenal columnar epithelium, and the function of this protein may be to inform the absorptive cells of the iron status of the body as observed in other organs.

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Authors and Affiliations

  1. The Second Department of Internal Medicine, Osaka Medical College, 2-7, Daigaku-machi, 569, Takatsuki, Osaka, Japan

    Hirofumi Miyoshi MD, Kiyoshi Ashida MD, Ichiro Hirata MD, Saburo Ohshiba MD & Tsuguya Naitoh PhD

  2. The Division of Central Research Laboratory, Osaka Medical College, 2-7, Daigaku-machi, 569, Takatsuki, Osaka, Japan

    Hirofumi Miyoshi MD, Kiyoshi Ashida MD, Ichiro Hirata MD, Saburo Ohshiba MD & Tsuguya Naitoh PhD

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  1. Hirofumi Miyoshi MD
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  2. Kiyoshi Ashida MD
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  5. Tsuguya Naitoh PhD
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Miyoshi, H., Ashida, K., Hirata, I. et al. Transferrin is not involved in initial uptake process of iron in rat duodenal mucosa. Digest Dis Sci 40, 1484–1490 (1995). https://doi.org/10.1007/BF02285196

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  • DOI: https://doi.org/10.1007/BF02285196

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