Journal of Neurocytology

, Volume 25, Issue 1, pp 645–657 | Cite as

Ultrastructural study on the interaction of native and cationized albumin-gold complexes with mouse brain microvascular endothelium

  • A. W. Vorbrodt
  • D. H. Dobrogowska
  • A. S. Lossinsky


The main objective of this ultrastructural study was to gain insights into the cellular mechanisms responsible for the enhanced brain uptake of blood-borne cationized albumin observed by several authors utilizing quantitative methodology. Mice were injected intravenously or into the common carotid artery (in vivo experiments) or perfusedin situ with solutions of native or cationized bovine serum albumin complexed with colloidal gold (BSA-G or cBSA-G respectively). The results indicate that: (1) the main drawbacks ofin vivo experiments are very intense phagocytosis of the tracer particles by Kupffer cells located in the liver sinusoids and also escape of the tracer through capillaries of skeletal and heart muscles. This results in a rapid decline of the concentration and disappearance of the circulating tracer particles; (2) BSA-G and cBSA-G bothin vivo (up to 30 min circulation) or after perfusionin situ (up to 15 min) do not cross the wall of brain microvessels representing the blood-brain barrier; (3) enhanced entrance of cationized albumin into the brain occurs through fenestrated endothelium of the capillaries located in the examined circumventricular organs (median eminence and neurohypophysis). Although BSA-G is also transported by these fenestrated capillaries, this process is evidently less intense than in the case of cBSA-G; (4) the enhanced passage of cBSA-G across fenestrated capillaries occurs mainly via vesicular transport (adsorptive transcytosis), through transendothelial channels and eventually through interendothelial junctional clefts; (5) the fenestrated capillaries of the choroid plexus appear to be less permeable for both tracers than those located in the other circumventricular organs.


Colloidal Gold Tracer Particle Median Eminence Brain Uptake Vesicular Transport 
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Copyright information

© Chapman and Hall 1996

Authors and Affiliations

  • A. W. Vorbrodt
    • 1
  • D. H. Dobrogowska
    • 1
  • A. S. Lossinsky
    • 1
  1. 1.New York State Office of Mental Retardation and Developmental DisabilitiesInstitute for Basic Research in Developmental DisabilitiesStaten IslandUSA

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