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Automated chromatography of protected oligonucleotides with high separation efficiency

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Summary

During the total chemical synthesis of a structural gene for the human peptide hormone angiotensin II [1] some difficult problems in ion-exchange chromatography of protected oligodeoxyribonucleotides were solved using an adsorption and a chromatographic column in combination with an automatic gradient generator and gradienttermination controller. This arrangement allows a well-timed gradient termination and therefore the separation of substances with only a small difference in charge. On the other hand the automatic run of the chromatography of protected oligonucleotides reduces the time factor in oligonucleotide chemistry. This technique is applicable for ion-exchange chromatography in general.

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References

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Authors and Affiliations

  1. Institut für Organische Chemie und Biochemie der Universität, Martin-Luther-King-Platz 6, D-2000, Hamburg 13, West Germany

    H. Köster & R. Frank

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  1. H. Köster
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  2. R. Frank
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Köster, H., Frank, R. Automated chromatography of protected oligonucleotides with high separation efficiency. Chromatographia 9, 497–501 (1976). https://doi.org/10.1007/BF02282662

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  • DOI: https://doi.org/10.1007/BF02282662

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