Chromatographia

, Volume 20, Issue 2, pp 79–82 | Cite as

Application of pharmacia automated FPLC System and PepRPC HR 5/5 bonded phase column for determination of dansylated polyamines

  • T. Kremmer
  • M. Boldizsár
  • L. Holczinger
Originals

Summary

The Pharmacia Automatized FPLC System equipped with a PepRPC HR 5/5 bonded-phase column was tested with dansyl polyamines, toluene, dimethyl- and dibutylphthalate, using either isocratic conditions or linear gradient-elution program of methanol in water as the mobile phase. A simple and reproducible method is described for the quantiation of dansylated natural polyamines, such as putrescine, spermidine and spermine originating from P388/S leukemia cells. Comparing the data from analyses performed in parallel by the Automated FPLC/PepRPC HR 5/5, and a Hewlett-Packard Model 1084B/LiChrosorb RP-8 systems a linear correlation has been found, with a regression coefficient of r=0.974.

Key Words

Automated fast-performance LC system Pep RPC HR 5/5 bonded phase column Apolar test compounds Dansyl polyamines, quantitation of P388/S leukemia cells 

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Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1985

Authors and Affiliations

  • T. Kremmer
    • 1
  • M. Boldizsár
    • 1
  • L. Holczinger
    • 1
  1. 1.Department of Biochemistry, Research Institute of Oncopathologynational Oncological InstituteBudapestHungary

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