, Volume 20, Issue 2, pp 79–82 | Cite as

Application of pharmacia automated FPLC System and PepRPC HR 5/5 bonded phase column for determination of dansylated polyamines

  • T. Kremmer
  • M. Boldizsár
  • L. Holczinger


The Pharmacia Automatized FPLC System equipped with a PepRPC HR 5/5 bonded-phase column was tested with dansyl polyamines, toluene, dimethyl- and dibutylphthalate, using either isocratic conditions or linear gradient-elution program of methanol in water as the mobile phase. A simple and reproducible method is described for the quantiation of dansylated natural polyamines, such as putrescine, spermidine and spermine originating from P388/S leukemia cells. Comparing the data from analyses performed in parallel by the Automated FPLC/PepRPC HR 5/5, and a Hewlett-Packard Model 1084B/LiChrosorb RP-8 systems a linear correlation has been found, with a regression coefficient of r=0.974.

Key Words

Automated fast-performance LC system Pep RPC HR 5/5 bonded phase column Apolar test compounds Dansyl polyamines, quantitation of P388/S leukemia cells 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. [1]
    J. Janne, H. Pösö, A. Raina, Biochim. Biophys. Acta473, 241 (1978).Google Scholar
  2. [2]
    H. G. Williams-Ashman, Z. N. Canellakis, Perspectives in Biology and Medicine22, 421 (1979).Google Scholar
  3. [3]
    O. Heby, Differentiation19, 1 (1981).Google Scholar
  4. [4]
    G. Scalabrino, M. E. Ferioli, in “Advances in Cancer Research”,G. Klein, S. Weinhouse, eds., Academic Press, New York, Part I: 35, 151 (1981, Part 11: 36, 1 (1982).Google Scholar
  5. [5]
    M. M. Abdel-Monem, K. Ohno, N. E. Newton, C. E. Weeks, in “Advances in Polyamine Research”,R. A. Campbell et al., eds., Raven Press, New York, 1978; p. 37.Google Scholar
  6. [6]
    N. Seiler, in: “Polyamines in Biomedical Research”,J. M. Gaugas, ed., J. Wiley and Sons, Chichester, New York, Brisbane, Toronto, 1980, p. 435.Google Scholar
  7. [7]
    L. J. Marton, in: “Liquid Chromatography in Clinical Analysis”,P. M. Kabra, L. J. Marton, eds., Humana Press, Clitfton, NJ 1981: p. 445.Google Scholar
  8. [8]
    N. Seiler, B. Knödgen, F. Eisenbeiss, J. Chromatogr.145, 29 (1978).Google Scholar
  9. [9]
    Y. Saeki, N. Uehara, S. Shirakawa, J. Chromatogr.,145, 221 (1978).Google Scholar
  10. [10]
    N. D. Brown, R. B. Sweet, J. A. Kintzios, H. D. Cox, B. P. Doctor, J. Chromatogr.164, 35 (1979).Google Scholar
  11. [11]
    T. Kremmer, L. Holczinger, M. Boldizsár, L. Selmeci, S. Bardócz, J. Chromatogr.286, 371 (1984).Google Scholar
  12. [12]
    Pharmacia FPLC System, Pharmacia Fine Chemical AB, Uppsala, Västra Aros, 1984-1.Google Scholar
  13. [13]
    J. Richey, American Laboratory, Oct. 1 (1982).Google Scholar
  14. [14]
    RPC of Peptides, Polypeptides and Protein with the Pharmacia FPLC System. Pharmacia Fine Chemicals AB, Uppsala, 1984.Google Scholar
  15. [15]
    R. E. Majors, J. Chromatogr. Sci.15, 333 (1977).Google Scholar
  16. [16]
    R. E. Majors, J. Chromatogr. Sci.18, 488 (1980).Google Scholar
  17. [17]
    F. L. Vandemark, J. L. DiCesare, Chromatography Newsietter9, 33 (1981).Google Scholar
  18. [18]
    A. P. Goldberg, Anal. Chem.54, 342 (1982).Google Scholar
  19. [19]
    J. S. Landy, J. L. Ward, J. G. Dorsey, J. Chromatogr. Sci.21, 49 (1983).Google Scholar
  20. [20]
    I. Pigulla, E. Röder, Fresenius Z. Anal. Chem.293, 404 (1978).Google Scholar
  21. [21]
    M. Kai, T. Ogata, K. Haraguchi, Y. Ohkura, J. Chromatogr.163, 151 (1979).Google Scholar
  22. [22]
    T. Kremmer, L. Seimeci, S. Bardócz, L. Holczinger, S. Bálint, Exp. Cell Biol.52, 279 (1984).Google Scholar

Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1985

Authors and Affiliations

  • T. Kremmer
    • 1
  • M. Boldizsár
    • 1
  • L. Holczinger
    • 1
  1. 1.Department of Biochemistry, Research Institute of Oncopathologynational Oncological InstituteBudapestHungary

Personalised recommendations