Summary
The essential oils have been isolated from tobacco leaves using a simple gas co-distillation apparatus. The method involves co-distillation with water under the continuous stream of an inert gas with subsequent single solvent extraction of the distillate. The extract is analysed on GC and GC/MS in splitless injection mode without further concentration. The method was evaluated for reproducibility using n-octadecane as an internal standard. The distillation-extraction procedure was reporducible with an average deviation of ±3% for the major constituents. The present method was applied to the analyses of the essential oils of other agricultural products.
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Dedicated to Dr. L. S. Ettre on the occasion of his 60th birthday.
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Kim, K.R., Zlatkis, A., Park, J.W. et al. Isolation of essential oils from tobacco by gas co-distillation/solvent extraction. Chromatographia 15, 559–563 (1982). https://doi.org/10.1007/BF02280374
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DOI: https://doi.org/10.1007/BF02280374