Abstract
The role of lateral root nodules in N2 fixation and the relationships between total shoot N and several traits which influence or control N2 fixation in common bean (Phaseolus vulgaris L.)i.e., acetylene reduction value, specific nodule activity, leghemoglobin concentration, total leghemoglobin and nodule mass, were investigated in field studies. Significant variation among bean lines was observed for all the traits measured. Lines varied for the proportion of total N accumulated up to the R3 growth state, thus measurements of total shoot N near maturity (e.g., R7) provided a better estimate of total N2 fixation than measurements taken at an early growth stage. Nodule mass was correlated with acetylene reduction and total leghemoglobin, and total leghemoglobin was correlated with acetylene reduction value. Total shoot N at R7 was correlated with seasonal means of nodule mass and number, acetylene reduction value and total leghemoglobin. For all traits except total leghemoglobin, values for lateral roots were more highly correlated with total shoot N than were values for either crown roots or the whole root system. Seed yield was most highly correlated with nodule mass of the lateral roots. These results will be useful in devising breeding strategies for improved N2 fixation of the host plant.
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Attewell J and Bliss F A 1985 Host plant characteristics of common bean lines selected using indirect measures of N2 fixation.In Nitrogen Fixation Research Progress. Eds. H J Evans, P J Bottomley and W E Newton. pp 3–9. Martinus Nijhoff Publishers, Boston.
Burris R H and Wilson P W 1957 Methods for measurement of nitrogen fixation.In Methods in Enzymology. Vol. III. Eds. S P Colwick and N O Kaplan. pp 355–366. Academic Press, N. Y.
Franco A A and Dobereiner J 1967 Especifidade hospedleira na siembose com Rhizobium — feijae influencia de diferentes nutrientes. Pesqui. Agropecu. Bras. 2, 467–474.
Graham P H and Rosas J C 1977 Growth and development of indeterminate bush and climbing cultivars ofPhaseolus vulgaris L. inoculated with Rhizobium. J. Agric. Sci. Camb. 88, 503–508.
Hardy R W F, Holsen R D, Jackson E K and Burns R C 1968 The acetylene-ethylene assay for N2 fixation: Laboratory and field evaluation. Plant Physiol. 43, 1185–1207.
Johnson H S and Hume D J 1973 Comparisons of nitrogen fixation estimates in soybeans by nodule weight, leghemoglobin content, and acetylene reduction. Can. J. Microbiol. 19, 1165–1168.
Lawn R J and Brun W A 1974 Symbiotic nitrogen fixation in soybeans. I. Effect of photosynthetic source-sink manipulations. Crop. Sci. 14, 11–16.
McFerson J R 1983 Genetic and breeding studies of dinitrogen fixation in common bean,Phaseolus vulgaris L. Ph.D. Thesis, Univ. of Wisconsin, Madison.
Nash D T and Schulman H M 1976 leghemoglobin and nitrogenase activity during soybean root nodule development. Can. J. Bot. 54, 2790–2797.
Patterson T G and LaRue T A 1983 Nitrogen fixation by soybeans: Seasonal and cultivar effects and comparison of estimates. Crop Sci. 23, 488–492.
Pladys D and Rigaud J 1985 Senesence in french bean nodules: Occurrence of different proteolytic activities.Physiol. Plant. 63, 43–48.
Rennie R J and Kemp G A 1981 Selection of dinitrogen fixing ability inPhaseolus vulgaris L. at two low-temperture regimes. Euphytica 30, 87–95.
Rennie R J and Kemp G A 1983 Nitrogen fixation in field beansPhaseolus vulgaris quantified by nitrogen-15 isotope dilution. 2. Effect of cultivars of beans. Agron. J. 75, 645–649.
Rennie R J and Kemp G A 198415N-determined time course for N2 fixation in two cultivars of field bean. Agron. J. 76, 146–154.
Ruschel A P, Vose P B, Matsui E, Victoria R L and Saito S M T 1982 Field evaluation of nitrogen fixation and nitrogen utilization byPhaseolus vulgaris bean varieties determined by nitrogen-15 isotope dilution. Plant and Soil 65, 397–408.
Schiffman J 1982 Biological and agronomic aspects of legume inoculation in Israel. Isr. J. Bot. 31, 265–281.
Schiffman J and Lobel R 1970 Hemoglobin determination and its value as an early indication of peanut rhizobium efficiency. Plant and Soil 33, 501–512.
Schweitzer L E and Harper J E 1985 Leaf nitrate reductase D-ribulose-1, 5-biophosphate carboxylase, and root nodule development of genetic male-sterile and fertile soybean isolines. Plant Physiol. 78, 61–65.
St. Clair D A, Wolyn D K 4 J, DuBois J, Burris R H and Bliss F A 1988 a field comparison of N2 fixation determined with15N-depleted ammonium sulfate and15N-enriched ammonium sulfate in selected inbred backcross lines of common bean. Crop Sci. 28, 773–778.
Swaraj K and Garay O P 1977 The effect of aging on the leghemoglobin of cowpea nodules. Physiol. Plant. 39, 185–189.
Westermann D T and Kolar J J 1978 Symbiotic N2(C2H2) fixation by bean. Crop Sci. 18, 986–990.
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Wolyn, D.J., Attewell, J., Ludden, P.W. et al. Indirect measures of N2 fixation in common bean (Phaseolus vulgaris L.) under field conditions: The role of lateral root nodules. Plant Soil 113, 181–187 (1989). https://doi.org/10.1007/BF02280179
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DOI: https://doi.org/10.1007/BF02280179