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Determination of laidlomycin and 26-deoxylaidlomycin by high-performance liquid chromatography

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Summary

High performance liquid chromatography on an octadecyl silica column has been used to determine laidlomycin: 26-deoxylaidlomycin ratios and to determine the concentration of both compounds by standard addition in samples prepared from fermentation broths ofStreptoverticillium olivoreticuli. A refractive index detector was preferred to an ultraviolet detector owing to the presence of UV-absorbing impurities which could not be completely separated from the substances of interest. Linear relationships were obtained from the calibration data. The coefficient of variation for the estimation of ratio and concentration of the compounds determined was better then 5%. The estimated limit of detection for both substances was about 50μg·ml−1.

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References

  1. F. Kitame, K. Utsushikawa, T. Kohama, T. Saito, M. Kukuchi, N. Ishida, J. Antib.27 (11), 884 (1974).

    Google Scholar 

  2. F. Kitame, N. Ishida, J. Antib.29 (7), (1976).

  3. U. Graefe, R. Schlegel, C. Stengel, W. Ihn, L. Radics, J. Basic Microbiol.29, 149 (1989).

    Google Scholar 

  4. J. W. Westley, Adv. Appl. Microbiol.22, 177 (1977).

    Google Scholar 

  5. U. Graefe, R. Schlegel, C. Stengel, W. Ihn, J. Wesner, Proceedings of 2nd International Symposium on New Bioactive Metabolites from Microorganisms, page 126, May 2–7, 1988, Gera, GDR.

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Authors and Affiliations

  1. Department of Biogenesis of Natural Substances, Institute of Microbiology, Czechoslovak Academy of Sciences, Vídeňská 1083, 142 20, Prague 4, Czechoslovakia

    M. Beran & J. Zima

  2. Department of Antibiotics, Central Institute of Microbiology and Experimental Therapy, Academy of Sciences, Beutenbergstraße 11, 6900, Jena, Germany

    K. Dornberger, C. Stengel & U. Graefe

Authors
  1. M. Beran
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  2. R. Zielke
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  3. K. Dornberger
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  4. C. Stengel
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  5. J. Zima
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  6. U. Graefe
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Beran, M., Zielke, R., Dornberger, K. et al. Determination of laidlomycin and 26-deoxylaidlomycin by high-performance liquid chromatography. Chromatographia 31, 603–605 (1991). https://doi.org/10.1007/BF02279483

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  • DOI: https://doi.org/10.1007/BF02279483

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