Abstract
The effects of different binary solvents on the retention behaviour of some steroids in thin-layer chromatography on alumina were studied. The slope of the linear relationship between the retention constant of the steroid and the logarithm of the volume fraction fo the polar component in the binary solvent mixture depends predominantly on the diluent. Linear relationships between the axis intercepts and slopes of particular steroids exists for all chromatographic systems examined. The slope of this relationships is a function of the retention constant of the steroid hydroxyl group.
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Petrović, S.M., Kolarov, L.A. & Petrović, J.A. Role of the solvent in thin-layer chromatography of steroids on alumina. Chromatographia 18, 37–40 (1984). https://doi.org/10.1007/BF02279463
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DOI: https://doi.org/10.1007/BF02279463