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Indole-3-acetic acid and indole-3-butyric acid in tissues of carrot inoculated withAgrobacterium rhizogenes

  • Ephraim Epstein
  • Scott J. Nissen
  • Ellen G. Sutter
Article

Abstract

The role of auxins in induction of roots byAgrobacterium rhizogenes was studied in carrot root disks. Transformed roots were produced on root disks by inoculation withA. rhizogenes, A4. Measurement of indole-3-acetic acid (IAA) by gas chromatography-mass spectrometry (GC-MS) indicated that there was a significant increase in the concentration of IAA in transformed callus and induced roots compared with initial IAA concentrations in carrot disks. Indole-3-butyric acid (IBA) was found to occur naturally in carrot roots. The presence of IBA, a potent root inducer, must be taken into account when assessing the role of auxin during transformation and induction of roots byA. rhizogenes.

Keywords

Hairy Root Adventitious Root Formation Agrobacterium Rhizogenes Carrot Root Hairy Root Induc 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag New York Inc. 1991

Authors and Affiliations

  • Ephraim Epstein
    • 1
  • Scott J. Nissen
    • 2
  • Ellen G. Sutter
    • 3
  1. 1.Department of HorticultureVolcani CenterBet Dagan
  2. 2.Department of AgronomyUniversity of NebraskaLincolnUSA
  3. 3.Department of PomologyUniversity CaliforniaDavisUSA

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