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Relationship between iron, calcium and phosphate during experimental cutaneous calcinosis

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Abstract

Calcification of the skin (calciphylaxis) induced by the subcutaneous administration of iron chloride to dihydrotachysterol (DHT)-sensitized rats is preceded by the selective deposition of iron (visible with electron microscopic techniques) on the surface of collagen fibrils. Electron probe X-ray microanalysis indicates that the injectionof iron alone suffices to increase local concentrations of calcium and phosphorus; however, apatite formation occurs only in animals pretreated with DHT. The amount and degree of crystallinity of the inorganic material is very similar on the sixth and thirtieth day after the beginning of the experiment.

Résumé

La calcification de la peau (calciphylaxie) provoquée par l'administration sous-cutanée de chlorure de fer chez des rats sensibilisés par le dihydrotachystérol (DHT) est précédée par une déposition sélective de fer (décelable à la microscopie électronique) à la surface des fibres collagènes.

Les analyses à la microsonde prouvent que les taux de calcium et de phosphore sont augmentés à l'endroit de l'injection du chlorure de fer même sans l'administration préalable de DHT; cependant, l'apatite ne se forme que chez les animaux prétraités par le DHT.

La quantité et le degré de cristallinité de la matière inorganique sont presque identiques au sixième et au trentième jour de l'expérience.

Zusammenfassung

Durch elektronenmikroskopische Untersuchungen wurde festgestellt, daß die durch subcutane Verabreichung von Eisenchlorid bei Dihydrotachysterin-sensibilisierten Ratten herbeigeführte Hautverkalkung durch eine selektive Eisenablagerung auf der Oberfläche der Kollagenfibrillen eingeleitet wird. Die Röntgenmikroanalyse zeigt an, daß die lokale Calciumund Phosphorkonzentration bereits nach Eiseninjektion allein ansteigt, während Apatitbildung nur in den mit DHT vorbehandelten Tieren erfolgt. Ausmaß und Menge der Kristallisation sind am 6. und 30. Tage nach Versuchsbeginn fast gleich.

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Authors and Affiliations

  1. Institut de Médecine et de Chirurgie expérimentales, Université de Montréal, Montreal, Canada

    G. Gabbiani M.D.

  2. Institut de Morphologie, Université de Genève, Geneva, Switzerland

    M. C. Badonnel & C. A. Baud

Authors
  1. G. Gabbiani M.D.
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  2. M. C. Badonnel
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  3. C. A. Baud
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Gabbiani, G., Badonnel, M.C. & Baud, C.A. Relationship between iron, calcium and phosphate during experimental cutaneous calcinosis. Calc. Tis Res. 4, 224–230 (1969). https://doi.org/10.1007/BF02279125

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  • DOI: https://doi.org/10.1007/BF02279125

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