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Chromatographia

, Volume 35, Issue 1–2, pp 93–96 | Cite as

Purification of125I-labelled aprotinin by hydrophobic interaction chromatography

  • G. Raspi
  • A. Lo Moro
  • M. Spinetti
Originals

Summary

125I-aprotinin has been prepared and purified by affinity chromatography in combination with hydrophobic interaction chromatography (HIC). The method allows the preparation of the labelled inhibitor practically carrier-free, in saline solution without organic modifier and the isolated compound retained inhibition activity. Recovery of radioactive material was virtually quantitative, without substantial accumulation of radioactivity on the column. Compared with currently used procedures, the method is more reliable because the labelled polypeptide is collected by following its specific chromatographic signal.

Key Words

Liquid chromatography Bovine pancreatic trypsin inhibitor 125I-labelled compounds 

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References

  1. [1]
    E. Reich, D. Rifkin, E. Shaw, eds., “Proteases and Biological Control”. Cold Spring Harbor Lab., Cold spring Harbor, New York, 1975.Google Scholar
  2. [2]
    J. W. Ryan, Methods Enzymol.,163, 160 (1988).PubMedGoogle Scholar
  3. [3]
    H. Fritz, G. Wunderer Arzneim.-Forsch./Drug Res.,33, 479 (1983).Google Scholar
  4. [4]
    R. Geiger, in: Methods of Enzymatic Analysis (H. U. Bergmeyer, ed.) 3rd ed., vol. 5, p. 129. Verlag Chemie, Weinheim (1984).Google Scholar
  5. [5]
    W. M. Hunter, F. C. Greenwood, Nature (London),194, 495 (1962).Google Scholar
  6. [6]
    F. C. Greenwood, W. M. Hunter, J. S. Glover, Biochem. J.,89, 114 (1963).PubMedGoogle Scholar
  7. [7]
    G. Raspi, A. Lo Moro, M. Spinetti Ann. Chim. (Rome),77, 525 (1987).Google Scholar
  8. [8]
    G. Raspi, A. Lo Moro, M. Spinetti, M. Molinari Analyst,114, 101 (1989).CrossRefPubMedGoogle Scholar
  9. [9]
    A. Vinther, S. Bjorn, H. H. Sorensen, H. Soeberg, J. Chromatogr.,516, 175 (1990).CrossRefPubMedGoogle Scholar

Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1993

Authors and Affiliations

  • G. Raspi
    • 1
  • A. Lo Moro
    • 1
  • M. Spinetti
    • 1
  1. 1.Dipartimento di Chimica e Chimica IndustrialeUniversità di PisaPisaItaly

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