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Secretin and cholecystokinin activities in extracts of sheep intestine

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Abstract

Extracts of sheep intestine prepared to contain secretin were readily obtained, reacted with antibodies raised to pure natural porcine secretin, were potent stimulants of pancreatic exocrine secretion of the anaesthetised rat and failed to contract sheep gall bladder in vitro. In contrast extracts prepared to contain cholecystokinin (CCK) caused contraction of sheep gall bladder in vitro but were more difficult to obtain than those of secretin due to a contaminant green pigment. The experiments provide biological and immunological evidence for the existence of secretin in sheep intestine. The demonstration of CCK activity on sheep gall bladder by separate extracts is indicative of the presence of CCK in the sheep but this awaits confirmation by immunological means.

Zusammenfassung

Schafdarmextrakte mit Sekretininhalt lassen sich ohne Schwierigkeiten herstellen; sie reagieren mit Antikörpern, die auf reines, natürliches Sekretin ansprechen, stellen einen wirksamen Auslöser für pankreatisch-exokrine Sekretion in betäubten Ratten dar und rufen keine Kontraktionen an Schafgallenblasen in vitro hervor. Andererseits vermögen Extrakte, die auf einer Methode zur Anreicherung von Cholezystokinin (CCK) beruhen, Kontraktionen an Schafgallenblasen in vitro zu erzeugen; sie waren jedoch wegen der Anwesenheit eines grünen Pigments schwieriger zu erhalten als die Extrakte mit Sekretingehalt. Die Experimente weisen auf biologischem und immunologischem Wege auf die Existenz von Sekretin im Verdauungstrakt des Schafes. Der Wirksamkeitsnachweis von CCK auf die Schafgallenblase durch separate Extrakte deutet auf Vorkommen von CCK im Schaf hin, doch bleibt der immunologische Nachweis abzuwarten.

Resumé

Des extraits d'intestin de mouton contenant de la secretine obtenus en quantité, réagirent avec des anticorps de secretine naturelle, pure de porc, stimulèrent vivement les secretions exocrine du pancréas de rats anesthesiés et ne réussissent pas à contracter en vitro le vessicule bilaire de mouton.

Bien que plus difficile à obtenir à cause de contamination par des pigments verts, des extraits préparés afin de contenir de la cholecystokinine (CCK) provoquèrent en vitro des contractions de vesicule bilaire de mouton.

Ces expériences fournissent la preuve biologique et immunologique de l'existence de la secretine dans l'intestin du mouton. L'activité du CCK sur le vesicule bilaire de mouton que nous avons démonstrée paraît indiquer la présence de CCK dans le mouton, mais nous en attendons la preuve par des moyens immunologiques.

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Authors and Affiliations

  1. Department of Biological Sciences, University of Waikato, Hamilton, (New Zealand)

    G. W. Reynolds & L. M. McLeay

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  1. G. W. Reynolds
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  2. L. M. McLeay
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Reynolds, G.W., McLeay, L.M. Secretin and cholecystokinin activities in extracts of sheep intestine. Vet Res Commun 4, 53–58 (1980). https://doi.org/10.1007/BF02278480

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