Plant and Soil

, Volume 52, Issue 4, pp 527–536 | Cite as

Lime-induced chlorosis inPinus radiata

  • George Nakos


Addition of marl (CaCO3) and/or manure to an acid soil in pots caused ‘lime-induced’ chlorosis inPinus radiata seedlings, especially after excessive irrigation for 15 days. Chlorotic symptoms and their intensity were found to be related more to soil moisture and to the HCO3 concentrations, than to percentage of free CaCO3, in the soil mixtures.

Comparative chemical analysis showed lower total Fe and Mn concentrations and higher concentrations of cations and organic anions in the needles of seedlings with chlorotic symptoms than in the needles of healthy ones.

Key Words

Greece Lime-induced chlorosis Pinus radiata 


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  1. 1.
    Biddulph, O. and Woodbridge, C. G. 1952 The uptake of phosphorus by bean with particular reference to the effects of iron. Plant Physiol.27, 431–444.Google Scholar
  2. 2.
    Boxma, R. 1972 Bicarbonate as the most important soil factor in lime-induced chlorisis in the Netherlands. Plant and Soil37, 233–243.Google Scholar
  3. 3.
    Brown, J. C. and Ambler, J. E. 1974 Iron-stress response in tomato (Lycopersicum esculentum). 1. Sites of Fe reduction, absorption and transport. Physiol. Plant31, 221–224.Google Scholar
  4. 4.
    Chino, M. and Mitsu, S. 1967 Effects of heavy metal excesses on the absorption, translocation and distribution of59Fe in rice plants. 2. J. Sci. Soil Manure, Tokyo38, 255–259.Google Scholar
  5. 5.
    Day, G. M. and Robbins, W. R. 1950 Observations on the growth of red spruce in sand culture. J. For.48, 689–692.Google Scholar
  6. 6.
    Dekock, P. C. and Morrison, R. J. 1958 The metabolism of chlorotic leaves 2. Organic acids. Biochem. J.70, 272–277.Google Scholar
  7. 7.
    DeWit, C. T., Dijkshoorn, W. and Noggle, J. C. 1963 Ionic balance and growth of plants. Versl. Landbouwkd. Onderz.69, 15.Google Scholar
  8. 8.
    Dijkshoorn, W. and Van Wijk, A. L. 1967 The sulfur requirements of plants as evidenced by the sulfur-nitrogen ratio in the organic matter. A review of published data. Plant and Soil26, 129–157.Google Scholar
  9. 9.
    Evans, E. J., Mack, A. R. and Dekker, A. J. 1968 Influence of temperature on the availability of iron to an oat crop. Can. J. Soil Sci.48, 185–192.Google Scholar
  10. 10.
    Iljin, W. S. 1951 Metabolism of plants growth affected with lime-induced chlorosis (calciose). II. Organic acids and carbohydrates. Plant and Soil3, 339–351.Google Scholar
  11. 11.
    Jones R. 1972 Comparative studies of plant growth and distribution in relation to waterlogging. V. The uptake of iron and manganese by dune and dune slack plants. J. Ecol.60, 131–139.Google Scholar
  12. 12.
    Jones, H. E. and Etherington, J. R. 1970 Comparative studies of plant growth and distribution in relation to waterlogging. I. Survival ofErica cinerea andE. tetralix. I. and its apparent relationship to iron and manganese uptake in waterlogged soil. J. Ecol.58, 487–496.Google Scholar
  13. 13.
    Nakos, G. 1975 Seasonal changes in foliar nutrient concentrations of forest species in the nursery: I. Conifers, Forest Res. Institute Publ. No.73, 24 pp. (English summary).Google Scholar
  14. 14.
    Nakos, G. 1975 Methods of soil and plant tissue analysis. For. Res. Inst. PublIII(2), 71–100. (In Greek).Google Scholar
  15. 15.
    Nelson, L. E. and Selby, R. 1974 The effect of nitrogen sources and iron levels on the growth and composition of sitka spruce and scots pine. Plant and Soil41, 573–588.Google Scholar
  16. 16.
    Place, G. A. 1969 Relationship of iron, manganese and bicarbonate to chlorosis of rice grown in calcareous soil. Rep. Ser. Ark. Agric. Exp. Stn175, 21 pp.Google Scholar
  17. 17.
    Pierce, E. C. and Appleman, C. O. 1943 Role of ether soluble organic acids in the cation-anion balance in plants. Plant Physiol.18, 224–238.Google Scholar
  18. 18.
    Porter, L. K. and Thorne, D. W. 1955 Interrelation of carbon dioxide and bicarbonate ions in causing plant chlorosis. Soil Sci.79, 373–382.Google Scholar
  19. 19.
    Rutland, R. B. 1966 Some aspects of the effects of high concentrations of calcium carbonate and various orthophosphates on the absorption, translocation, and utilization of iron byChrysanthemum morifolium. Diss. Abstr.26, 4157–4158.Google Scholar
  20. 20.
    Rutland, R. B. and Bukovac, M. J. 1971 The effect of calcium bicarbonate on iron absorption and distribution byChrysanthemum morifolium, Ram. Plant and Soil35, 225–236.Google Scholar
  21. 21.
    Scott, C. W. 1960Pinus radiata. FAO forestry and forest products studies No.14, 328 pp.Google Scholar
  22. 22.
    Shoulder, E. and Czabator, F. J. 1965 Chlorosis in a southern pine nursery: a case study. Tree Planter's Notes71, 19–21.Google Scholar
  23. 23.
    Singh, H. G. 1970 Effect of sulfur in preventing the occurrence of chlorosis in peas. Agron. J.62, 708–711.Google Scholar
  24. 24.
    Su, L. T. and Miller, G. W. 1961 Chlorosis in higher plants as related to organic acid content. Plant Physiol.36, 415–420.Google Scholar
  25. 25.
    Wallace, A. and Lunt, O. R. 1960 Iron chlorosis in horticultural plants, a review. Proc. Am. Hortic. Sci.75, 819–841.Google Scholar
  26. 26.
    Wallace, A. and Mueller, R. T. 1976 Behavior of iron-inefficient plants when grown in combinations of calcareous and noncalcareous soils. Commun. Soil Sci. Plant Anal.7, 107–110.Google Scholar
  27. 27.
    Wallace, A., Romney, E. M. and Alexander, G. V. 1976 Lime-induced chlorosis caused by excess irrigation water. Commun. Soil Sci. Plant Anal.7, 47–49.Google Scholar
  28. 28.
    Wallace, A., Wood, R. A. and Soufi, S. M. 1976 Cation-anion balance in lime-induced chlorosis. Commun. Soil. Sci. Plant Anal.7, 15–26.Google Scholar
  29. 29.
    Wallace, A. 1971 The competative chelation hypothesis of lime-induced chlorosis.In Regulation of Micronutrient Status of Plants by chelation Agents and other Factors. Ed. A. Wallace. Los Angeles, 230–239 p.Google Scholar
  30. 30.
    Westfall, D. G., Anderson, W. B. and Hodges, R. J. 1971 Iron and zinc response of chlorotic rice grown on calcareous soils. Agron. J.65, 702–705.Google Scholar
  31. 31.
    Zech, W. 1970 Nadelanalytische untersuchungen über die kalkchlorose der Waldkiefer (Pinus silvestris). Z. Pflanzenernaehr. Bodenkd.125, 1–16.Google Scholar

Copyright information

© Martinus Nijhoff 1979

Authors and Affiliations

  • George Nakos
    • 1
  1. 1.Soil Science LaboratoryForest Research InstituteAthensGreece

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