Summary
Following the evaluation of the nutritional requirements for thein vitro propagation ofElaeagnus angustifolia, this actinorhizal species was routinely multiplied on MS, supplemented with 100 mM sucrose and 5 μM kinetin. On this medium, at a 3 week-interval, a multiplication rate of 5–10 was observed. A morphological variant occurred in culture (wet type) but it was converted into the normal type (pubescent type) by a passage on 1/2 macro MS and 1.5% agar. One hundred percent rooting was achieved in liquid medium containing 1/2 MS without growth regulators. The plantlets were transferred aseptically to a nitrogen-free artificial soil substrate and inoculated with pure cultures of differentFrankia strains which had been isolated from Elaeagnus, Shepherdia and Hippophae host plants. We thus ascertained that afterin vitro propagation, the plants retained their capacity to nodulate and sustain nitrogen fixation.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bonga J M 1982 Vegetative propagation in relation to juvenility, maturity and rejuvenation.In Tissue Culture in Forestry. Eds. J M Bonga and D J Durzan. Martinus Nijhoff/Dr W. Junk Publishers, The Hague. pp 387–412.
Brown C L and Sommer H E 1982 Vegetative propagation of dicotyledonous trees.In Tissue Culture in Forestry. Eds. J M Bonga and D J Durzan. Martinus Nijhoff/Dr W Junk Publishers, The Hague. pp 109–149.
David A 1982In vitro propagation of gymnosperms.In Tissue Culture in Forestry. Eds. J M Bonga and D J Durzan. Martinus Nijhoff/Dr W Junk Publishers, The Hague. pp 72–108.
Fessenden R J 1979 Use of actinorhizal plants for land reclamation and amenity planting in the U.S.A. and Canada.In Symbiotic Nitrogen Fixation in the Management of Temperate Forests. Eds. J C Gordon, C T Wheeler and D A Perry, Oregon State University, Corvalis OR, pp 403–419.
Funk D T, Schlesinger R C and Ponder F Jr 1979 Autumn-olive as a nurse plant for black walnut. Bot. Gaz. 140 (suppl.): S110-S114.
Jones O P, Hopgood M E and O'Farrell D 1977 Propagationin vitro of M26 apple rootstocks. J. Hort. Sci., 52, 235–238.
Lalonde M 1979 Techniques and observations of the nitrogen-fixingAlnus root nodule symbiosis.In Recent Advances in Biological Nitrogen Fixation. Ed. N S Subba Rao. Oxford and IBH Publication. New Delhi, pp 421–434.
Lalonde M and Calvert H E 1979 Production ofFrankia hyphae and spores as an infective inoculant forAlnus species.In Symbiotic Nitrogen Fixation in the Management of Temperate Forests. Eds. J C Gordon, C T Wheeler and D A Perry. Oregon State University, Corvallis, OR pp 95–110.
Lalonde M, Calvert H E and Pine S 1981 Isolation and use ofFrankia strains in actinorhizae formationIn Current Perspectives in Nitrogen Fixation. Eds. A H Gibson and W E Newton, Australian Academy of Science. Canberra, pp 296–299.
Mott R L 1981 Trees.In Cloning Agricultural Plantsvia in vitro Techniques. Ed. B V Conger. CRC Press, Boca Raton. pp 217–254.
Murashige T 1974 Plant propagation through tissue culture. Annu. Rev. Plant Phys. 25, 135–166.
Murashige T and Skoog F 1962 A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant. 15, 473–497.
Normand P 1982 Isolement, caractérisation et évaluation de souches deFrankia. Master Thesis, Université Laval, Québec, Qué, Canada. 137 p.
Périnet P and Lalonde M 1983In vitro propagation and nodulation of the actinorhizal host plantAlnus glutinosa (L.) Gaertn. Plant Sci. Lett. 29, 9–17.
Périnet P and Lalonde M 1983 Axenic nodulation ofin vitro propagatedAlnus glutinosa plantlets byFrankia strains. Can. J. Bot. 61, 2883–2888.
Romberger J A and Tabor C A 1971 ThePicea abies shoot apical meristem in culture. I. Agar and autoclaving effects. Am. J. Bot. 58, 131–140.
Steiner A A and Van Winden H 1970 Recipe for ferric salts of ethylenediaminetetraacetic acid. Plant Physiol. 46, 862–863.
Tremblay F M and Lalonde M 1984 Requirements forin vitro propagation of seven nitrogen fixingAlnus species. Plant Cell Tissue Organ Cult. 3, 189–199.
Tremblay F M, Nesme X and Lalonde M 1984 Selection and micropropagation of nodulating and non-nodulating clones ofAlnus crispa (Ait.) Pursh. Plant and Soil 78, 171–179.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Bertrand, L.J., Lalonde, M. In vitro propagation and nodulation byFrankia of actinorhizal Russian Olive (Elaeagnus angustifolia L.). Plant Soil 87, 143–152 (1985). https://doi.org/10.1007/BF02277655
Issue Date:
DOI: https://doi.org/10.1007/BF02277655