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Plant and Soil

, Volume 87, Issue 1, pp 31–42 | Cite as

Characterization and infectivity of a spontaneous variant isolated fromFrankia sp. WEY 0131391

  • Willie L. Parson
  • Linda R. Robertson
  • Carolyn V. Carpenter
Article

Summary

A spontaneous variant, obtained from aFrankia isolate fromAlnus rubra nodules, was compared with the parent strain with regard to infectivity, nitrogenase activity, and electrophoretic and immunological profiles. Both the parent and the variant strain were equally effective in inducing nodulation in seedlings ofA. rubra. All inoculated plants had an active nitrogenase system as measured by the acetylene reduction assay. Electrophoresis of whole cell homogenates on SDS-polyacrylamide slab gels showed similar electrophoretic profiles; however, the variant strain also exhibited striking differences in protein patterns that distinguish it from the parent strain. Immunological analysis of the originalFrankia strain and its variant revealed shared antigens as well as immunologically distinct antigenic determinants in the two strains. The variant strain exhibits a distinct morphology and growth patterns which remain stable after many passages through culture.

Key words

Acetylene reduction Alnus rubra Electrophoresis Endophyte Frankia Immunodiffusion 

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Copyright information

© Martinus Nijhoff Publishers 1985

Authors and Affiliations

  • Willie L. Parson
    • 1
  • Linda R. Robertson
    • 1
  • Carolyn V. Carpenter
    • 1
  1. 1.Department of BiotechnologyWeyerhaeuser Technology CenterTacomaUSA

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