Summary
A simple and sensitive ion chromatographic method for the estimation of tresyl groups on several activated supports (tresylated diol silica, tresylated Sepharose 4B and Tresyl-5PW) has been developed. The method involves the hydrolysis of tresyl groups on the supports followed by ion chromatographic determination of the liberated 2,2,2-trifluoroethanesulfonic acid. It is useful for the prediction of the immobilization efficiency of proteins.
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Ohta, T., Tairyo, Y. & Takitani, S. An ion chromatographic method for the estimation of tresyl groups on activated supports. Chromatographia 35, 321–324 (1993). https://doi.org/10.1007/BF02277518
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DOI: https://doi.org/10.1007/BF02277518