Plant and Soil

, Volume 90, Issue 1–3, pp 203–209 | Cite as

Studies of the adenylate and pyridine nucleotide pools during nitrogenase ‘switch-off’ inRhodospirillum rubrum

  • S. Nordlund
  • L. Höglund
Biochemistry and Physiology of Nitrogen Fixation


When ammonium ions are added to a nitrogen fixing culture ofRhodospirillum rubrum, nitrogenase activity decreases due to inactivation of the Fe-protein. We have studied the adenylate and pyridine nucleotide pools during switch-off using the sensitive bioluminescence method. Immediately after the addition of ammonium ions there is a decrease in the ATP pool which is quickly reversed and no change is seen during the switch-off period. The pyridine nucleotide pools also do not change significantly during the switch-off. Consequently we conclude that changes in the pools studied were not the signal promoting inactivation of the Fe-protein.

Key words

Nitrogen fixation Nucleotide pools Rhodospirillum rubrum Switch off 


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Copyright information

© Martinus Nijhoff Publishers 1986

Authors and Affiliations

  • S. Nordlund
    • 1
  • L. Höglund
    • 1
  1. 1.Department of BiochemistryArrhenius LaboratoryStockholmSweden

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