Plant and Soil

, Volume 90, Issue 1–3, pp 203–209 | Cite as

Studies of the adenylate and pyridine nucleotide pools during nitrogenase ‘switch-off’ inRhodospirillum rubrum

  • S. Nordlund
  • L. Höglund
Biochemistry and Physiology of Nitrogen Fixation

Summary

When ammonium ions are added to a nitrogen fixing culture ofRhodospirillum rubrum, nitrogenase activity decreases due to inactivation of the Fe-protein. We have studied the adenylate and pyridine nucleotide pools during switch-off using the sensitive bioluminescence method. Immediately after the addition of ammonium ions there is a decrease in the ATP pool which is quickly reversed and no change is seen during the switch-off period. The pyridine nucleotide pools also do not change significantly during the switch-off. Consequently we conclude that changes in the pools studied were not the signal promoting inactivation of the Fe-protein.

Key words

Nitrogen fixation Nucleotide pools Rhodospirillum rubrum Switch off 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Alef K, Arp D J and Zumft W G 1981 Nitrogensae switch-off by ammonia inRhodopseudomonas palustris: Loss under nitrogen deficiency and independence from adenylyation state of glutamine synthetase. Arch. Microbiol. 130, 138–142.Google Scholar
  2. 2.
    Arp D J and Zumft W G 1980 L-Methionine-SR-Sulfoximine as a probe for the role of glutamine synthetase in nitrogenase switch-off by ammonia and glutamine inRhodopseudomonas palustris. Arch. Microbiol. 134, 17–22.Google Scholar
  3. 3.
    Brown C M and Herbert R A 1977 Ammonia assimilation in members of the Rhodospirillaceae. FEMS Microbiol. Lett. 1, 43–46.Google Scholar
  4. 4.
    Carithers R P, Yoch D C and Arnon D I 1979 Two forms of nitrogenase from the photosynthetic bacteriumRhodospirillum rubrum. J. Bacteriol. 137, 779–789.Google Scholar
  5. 5.
    Falk G, Johansson B C and Nordlund S 1981 The role of glutamine synthetase in the regulation of nitrogenase activity (‘switch-off’ effect) inRhodospirillum rubrum. Arch. Microbiol. 132, 251–253.Google Scholar
  6. 6.
    Gest H, Kamen M D and Bregoff H M 1950 Studies on the metabolism of photosynthetic bacteria. J. Biol. Chem. 182, 155–170.Google Scholar
  7. 7.
    Hillmer P and Fahlbusch K 1979 Evidence for an involvement of glutamine synthetase in regulation of nitrogenase activity inRhodopseudomonas capsulata. Arch. Microbiol. 112, 213–218.Google Scholar
  8. 8.
    Johansson B C, Nordlund S and Baltscheffsky H 1983 Nitrogen fixation and ammonia assimilation.In The Phototrophic Bacteria. Ed. J G Ormerod. pp 120–145, Blackwell Scientific Publications, Oxford.Google Scholar
  9. 9.
    Jones B L and Monty K J 1979 Glutamine as a feed back inhibitor of theRhodopseudomonas sphaeroides nitrogenase system. J. Bacteriol. 139, 1007–1013.Google Scholar
  10. 10.
    Kanemoto R H and Ludden P W 1984 Effect of ammonia, darkness and phenazine methosulfate on whole-cell nitrogenase activity and Fe protein modification inRhodospirillum rubrum. J. Bacteriol. 158, 713–720.Google Scholar
  11. 11.
    London J and Knight M 1966 Concentrations of nicotinamide nucleotide coenzymes in micro-organisms. J. Gen. Microbiol. 44, 241–254.Google Scholar
  12. 12.
    Lowry O H, Rosebrough N J, Farr A L and Randall R J 1951 Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193, 265–275.Google Scholar
  13. 13.
    Ludden P W and Burris R H 1976 Activating factor for the iron protein of nitrogenase fromRhodospirillum rubrum. Science 194, 424–426.Google Scholar
  14. 14.
    Ludden P W and Burris R H 1978 Purification and properties of nitrogenase fromRhodospirillum rubrum and evidence for phosphate, ribose and an adenine-like unit covalently bound to the iron protein. Biochem. J. 175, 251–259.Google Scholar
  15. 15.
    Ludden P W, Murrel S A, Pope M, Manemoto R, Dowling T E, Saari L L and Triplett E 1984 Regulation of nitrogen fixation in photosynthetic bacteria.In Advances in Nitrogen Research. Eds. C Veeger and W E Newton. pp 181–187 Martinus Nijhoff/Dr W Junk Publishers, The Hague and Pudoc Wageningen.Google Scholar
  16. 16.
    Nagatani H, Shimizu M and Valentine R C 1971 The mechanism of ammonia assimilation in nitrogen fixing bacteria. Arch. Miccrobiol. 79, 164–175.Google Scholar
  17. 17.
    Neilson A H and Nordlund S 1975 Regulation of nitrogenase synthesis in intact cells ofRhodospirillum rubrum: Inactivation of nitrogen fixation by ammonia, L-glutamine and L-asparagine. J. Gen. Microbiol. 91, 53–62.Google Scholar
  18. 18.
    Nordlund S and Eklund R 1979 Nitrogen fixation inRhodospirillum rubrum—the switch-off effect. Third International Symposium on Photosynthetic Procaryotes, Oxford, England, abstract no B47.Google Scholar
  19. 19.
    Nordlund S and Eriksson U 1979 Nitrogenase fromRhodospirillum rubrum: relation between the ‘switch-off’ effect and the membrane component, hydrogen production and acetylene reduction with different ratios of the nitrogenase components. Biochim. Biophys. Acta 547, 429–437.Google Scholar
  20. 20.
    Nordlund S, Eriksson U and Baltscheffsky H 1977 Necessity of a membrane component for nitrogenase activity inRhodospirillum rubrum. Biochim. Biophys. Acta 462, 187–195.Google Scholar
  21. 21.
    Ohmori M and Hattori A 1978 Transient change in the ATP pool ofAnabaena cylindrica associated with ammonia assimilation. Arch. Microbiol. 117, 17–20.Google Scholar
  22. 22.
    Ormerod J G, Ormerod K S and Gest H 1961 Light-dependent utilization of organic compounds and photo-production of molecular hydrogen by photosynthetic bacterial; relationships with nitrogen metbolism. Arch. Biochem. Biophys. 94, 449–463.Google Scholar
  23. 23.
    Shick H-J 1971 Substrate and light dependent fixation of molecular nitrogen inRhodospirillum rubrum. Arch. Microbiol. 75, 89–101.Google Scholar
  24. 24.
    Sweet W J and Burris R H 1981 Inhibition of nitrogenase activity by NH4+ inRhodospirillum rubrum. J. Bacteriol. 145, 824–831.Google Scholar
  25. 25.
    Sweet W J and Burris R H 1982 Effects ofin vivo treatments on the activity of nitrogenase fromRhodospirillum rubrum. Biochim. Biophys. Acta 680, 17–21.Google Scholar
  26. 26.
    Yelton M M and Yock D C 1981 Nitrogen metabolism inRhodospirillum rubrum: characterization of glutamate synthase. J. Gen. Microbiol. 123, 335–342.Google Scholar
  27. 27.
    Yock D C 1980 Regulation of nitrogenase A and R concentrations inRhodopseudomonas capsulata by glutamine synthetase. Biochem. J. 187, 273–276.Google Scholar
  28. 28.
    Yoch D C and Cantu M 1980 Changes in the regulatory form ofRhodospirillum rubrum nitrogenase as influenced by nutritional and environmental factors. J. Bacteriol. 142, 899–907.Google Scholar
  29. 29.
    Zumft W G and Castillo F 1978 Regulatory properties of the nitrogenase fromRhodospirillum palustri. Arch. Microbiol. 117, 55–60.Google Scholar

Copyright information

© Martinus Nijhoff Publishers 1986

Authors and Affiliations

  • S. Nordlund
    • 1
  • L. Höglund
    • 1
  1. 1.Department of BiochemistryArrhenius LaboratoryStockholmSweden

Personalised recommendations