Summary
A new method for the extraction of basic drugs from whole blood based on the use of a buffered protein precipitating reagent at pH9, to form NiWO4 in biological fluids and a ternary organic extracting phase of density below 1 is proposed. The extract is analysed by high performance liquid chromatography using a Hypersil C8 column and detection at 230 nm; the mobile phases used consist of binary mixtures of acetonitrile and aqueous solutions containing 20 mmol/l ofn-propylamine at pH 5 andn-hexylamine at pH 4, as blockers of the free silanol groups. The proposed method was applied to the determination of 16 basic drugs including narcotics, analgesics, cocaine-related compounds and adulterants, as well as 8 benzodiazepines, in whole blood.
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References
R. D. McDowall, J. Chromatogr.492, 3 (1989).
M. R. Smith, Chemical Analysis in Complex Matrices, 1st. edn., Ellis Horwood Ltd., New York (1992), p. 288.
J. Clarke's, Isolation and Identification of Drugs, 2nd. edn., The Pharmaceutical Press, Pensylvania (1986) p. 1223.
T. J. Henry, D. C. Cannon, Clinical Chemistry. Principles and Technics, 2nd. ed., Harper & Row Pu. Inc., Maryland (1980) chap. 15.
M. Somogyi, J. Biol. Chem.160-1, 69 (1945).
G. A. E. van't Klooster, F. M. A. Woutersen-van Nijnanten, H. J. Kolker, J. Noordhoek, A. S. J. P. A. M. van Miert, J. Chromatogr.579, 158 (1992).
R. L. Miller, C. L. Devane, J. Chromatogr.570, 412 (1991).
D. Wiehelm, A. Kemper, J. Chromatogr.525, 218 (1990).
X. Chen, J. Franke, J. Wijsbeek, R. A. Zeeuw, J. Anal. Toxicol.16, 351 (1992).
C. Collins, J. Muto, V. Spiehler, J. Anal. Toxicol.16, 340 (1992).
C. Tanford, J. Amer. Chem. Soc.74-1, 211 (1952).
K. Kustin, R. F. Pasternak, E. M. Weinstock, J. Amer. Chem. Soc.,88, 4610 (1966).
J. Walker, R. Nakon, J. Amer. Chem. Soc.99, 8359 (1977).
J. C. W. Chien, J. Amer. Chem. Soc.100, 1310 (1978).
A. Turcant, A. Premol-Cabic, A. Cailleux, P. Allain, Clin. Chem.37-7, 1210 (1991).
S. D. Ferrara, L. Tedeschi, G. Frison, D. Castagna, J. Anal. Toxicol.16, 217 (1992).
A. Boukhabza, A. A. J. Lugnier, P. Kintz, P. Mangin, J. Anal. Toxicol.16, 351 (1991).
P. R. Poupolo, M. E. Pothier, S. A. Single, Clin. Chem.37-5, 701 (1991).
P. D. Whitter, P. L. Cary, J. Anal. Toxicol.10, 68 (1986).
G. R. Nakamura, E. L. Way, Anal. Chem.47, 775 (1975).
P. Jatlow, H. Nardim, Clin. Chem.36-8, 1436 (1990).
P. Campins, R. Herraez, A. Sevillano, J. Chromatogr.612, 245 (1993).
T. L. Pierce, A. G. W. Murray, W. Hope, J. Chromatogr.30, 443 (1992).
M. Mazhar, S. R. Binder, J. Chromatogr.497, 201 (1989).
I. S. Lurie, D. A. Cooper, R. F. X. Klein, J. Chromatogr.598, 59 (1992).
T. Ardvidsson, J. Chromatogr.439, 353 (1988).
G. G. Perez, J. H. Mendez, M. J. G. Martin, I1 Farmaco38, 383 (1988).
D. Chin Leach, M. A. Stadalins, J. S. Berus, L. R. Snyder, LC-GC International1–5, 22 (1988).
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Bernal, J.L., del Nozal, M.J., Rosas, V. et al. Extraction of basic drugs from whole blood and determination by high performance liquid chromatography. Chromatographia 38, 617–623 (1994). https://doi.org/10.1007/BF02277164
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DOI: https://doi.org/10.1007/BF02277164