, Volume 38, Issue 9–10, pp 609–612 | Cite as

Separation of L-(+)-ascorbic and D-(−)-isoascorbic acids on multimodal phenylpropanolamine-coated silica

  • S. Wongyai


A mixture of L-(+)-ascorbic acid and its epimer, D-(−)-isoascorbic acid, have been separated on a phenylpropanolamine-coated silica support using multimodal chromatography. The retention order of the diastereoisomers is the same as that found in reversed-phase mode in which ionic interaction is predominant. Applications of such columns to the analysis of L-(+)-ascorbic acid in an ascorbic acid injection formulation and an orange juice sample are vigen.

Key Words

Column liquid chromatography Multimodal chromatography L-(+)-ascorbic and D-(−)-isoascorbic acids 


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Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1994

Authors and Affiliations

  • S. Wongyai
    • 1
  1. 1.Faculty of PharmacyRangsit UniversityPhathum ThaniThailand

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