Separation of L-(+)-ascorbic and D-(−)-isoascorbic acids on multimodal phenylpropanolamine-coated silica
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A mixture of L-(+)-ascorbic acid and its epimer, D-(−)-isoascorbic acid, have been separated on a phenylpropanolamine-coated silica support using multimodal chromatography. The retention order of the diastereoisomers is the same as that found in reversed-phase mode in which ionic interaction is predominant. Applications of such columns to the analysis of L-(+)-ascorbic acid in an ascorbic acid injection formulation and an orange juice sample are vigen.
Key WordsColumn liquid chromatography Multimodal chromatography L-(+)-ascorbic and D-(−)-isoascorbic acids
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