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Anwendung der Hochdruck-Flüssigkeits-Chromatographie zur Analyse von Penicillinen und Cephalosporinen

Application of high-pressure liquid chromatography to the analysis of penicillins and cephalosporins

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Summary

A reversed phase liquid chromatography method for the analysis of some penicillins and cephalosporins of closely related structure is described. The influence of eluent pH and sodium chloride concentration on the resolution of ampicillin and epicillin is discussed. The methodology can be applied to the selective analysis of synthetically produced antibiotics and also to their pharmaceutical preparations. Because of its high sensitivity (10−7 g cm−3) the method can also be used for the analysis of penicillins in physiological fluids.

Zusammenfassung

Es wird eine Methode zur Analyse einiger Penicilline und Cephalosporine mit eng verwandter Struktur mittels reversed-phase Flüssigkeits-Chromatographie beschrieben. Der Einfluß des pH-Wertes sowie der Natriumchlorid-Konzentration der mobilen Phase auf die Auflösung des Ampicillins und Epicillins wird diskutiert. Das Verfahren kann zur selektiven Analyse synthetisch hergestellter Antibiotika sowie den entsprechenden pharmazeutischen Zubereitungen verwendet werden. Wegen der hohen Empfindlichkeit (10−1 g cm−3) kann die Methode auch zur Analyse von Penicillinen in physiologischen Flüssigkeiten verwendet werden.

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Authors and Affiliations

  1. Analytische Laboratorien der Sandoz AG, Deutschherrnstr. 15-23, D-8500, Nürnberg, Bundesrepublik Deutschland

    V. Hartmann & M. Rödiger

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  1. V. Hartmann
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  2. M. Rödiger
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Hartmann, V., Rödiger, M. Anwendung der Hochdruck-Flüssigkeits-Chromatographie zur Analyse von Penicillinen und Cephalosporinen. Chromatographia 9, 266–272 (1976). https://doi.org/10.1007/BF02276297

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  • DOI: https://doi.org/10.1007/BF02276297

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