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Control of column influence on the wide range pH gradient in ion-exchange chromatography

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Summary

A method for optimization of the mobile phase composition in ion exchange chromatography with a wide range external pH gradient (approx. over 5 pH units) has been formulated. It is shown that programming of various wide range pH profiles which are not impaired by buffer interactions of the mobile phase with sorbent is possible. Utilization of the wide range external pH gradient in cation-exchange chromatography of bovine haemoglobin is also demonstrated.

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Authors and Affiliations

  1. Institute of Analytical Chemistry, Czechoslovak Academy of Sciences, Veveří 97, 611 42, Brno, Czechoslavakia

    K. Slais

  2. Tessek Laboratory, Institute of Analytical Chemistry, Czechoslovak Academy of Sciences, Veveří 97, 611 42, Brno, Czechoslavakia

    Z. Friedl

Authors
  1. K. Slais
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  2. Z. Friedl
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Slais, K., Friedl, Z. Control of column influence on the wide range pH gradient in ion-exchange chromatography. Chromatographia 33, 231–236 (1992). https://doi.org/10.1007/BF02276188

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  • DOI: https://doi.org/10.1007/BF02276188

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