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Relationship between fluconazole sensitivity ofCandida albicans isolates from HIV positive patients and serotype, adherence and CD4+ lymphocyte count

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Abstract

The sensitivity to fluconazole, serotype and adherence to buccal epithelial cells of 46Candida albicans isolates was studied in vitro. Strains were isolated from 18 HIV-positive patients with variable CD4+ lymphocyte counts who were receiving long-term fluconazole therapy. Results showed that sensitivity to fluconazole correlated with serotype. Of the sensitive isolates 81.5 % were serotype A and 18.5 % serotype B, whereas of the resistant isolates 37.5 % were serotype A and 62.5 % serotype B. Sensitivity to fluconazole also correlated with adherence capacity, resistant isolates showing slightly less adherence to buccal epithelial cells than sensitive isolates. Weakly adherent isolates represented 87 % of resistant isolates and only 63 % of sensitive isolates. Resistant strains were isolated in highly immunocompromised patients. All strains isolated in patients with CD4+ lymphocyte counts above 200 lymphocytes/µl were sensitive, whereas intermediate and resistant strains were isolated in patients with counts below 200 lymphocytes/µl. Statistical analyses showed no correlation between adherence of isolates to buccal epithelial cells and CD4+ lymphocyte counts.

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Authors and Affiliations

  1. Laboratoire de Parasitologie-Immunologie, Unité de Formation et de Recherche des Sciences Pharmaceutiques, Faculté de Pharmacie, 15 Avenue Charles Flahault, F-34060, Montpellier Cedex, France

    C. Imbert-Bernard, A. Valentin, M. Mallié & J. M. Bastide

  2. Service de Maladies Infectieuses et Tropicales, Hôpital Gui de Chauliac, Centre Hospitalier Universitaire, F-34295, Montpellier Cedex 5, France

    J. Reynes

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  1. C. Imbert-Bernard
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  2. A. Valentin
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  3. J. Reynes
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  4. M. Mallié
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  5. J. M. Bastide
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Imbert-Bernard, C., Valentin, A., Reynes, J. et al. Relationship between fluconazole sensitivity ofCandida albicans isolates from HIV positive patients and serotype, adherence and CD4+ lymphocyte count. Eur. J. Clin. Microbiol. Infect. Dis. 13, 711–716 (1994). https://doi.org/10.1007/BF02276053

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